Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions.

Journal Article (Journal Article)

We demonstrate a multimodal approach that combines a pump-probe with confocal reflectance and multiphoton autofluorescence microscopy. Pump-probe microscopy has been proven to be of great value in analyzing thin tissue sections of pigmented lesions, as it produces molecular contrast which is inaccessible by other means. However, the higher optical intensity required to overcome scattering in thick tissue leads to higher-order nonlinearities in the optical response of melanin (e.g., two-photon pump and one-photon probe) that present additional challenges for interpreting the data. We show that analysis of pigment composition in vivo must carefully account for signal terms that are nonlinear with respect to the pump and probe intensities. We find that pump-probe imaging gives useful contrast for pigmented structures over a large range of spatial scales (100 μm to 1 cm), making it a potentially useful tool for tracking the progression of pigmented lesions without the need to introduce exogenous contrast agents.

Full Text

Duke Authors

Cited Authors

  • Wilson, JW; Degan, S; Gainey, CS; Mitropoulos, T; Simpson, MJ; Zhang, JY; Warren, WS

Published Date

  • May 2015

Published In

Volume / Issue

  • 20 / 5

Start / End Page

  • 051012 -

PubMed ID

  • 25415567

Pubmed Central ID

  • PMC4409034

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

Digital Object Identifier (DOI)

  • 10.1117/1.JBO.20.5.051012


  • eng

Conference Location

  • United States