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Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions.

Publication ,  Journal Article
Wilson, JW; Degan, S; Gainey, CS; Mitropoulos, T; Simpson, MJ; Zhang, JY; Warren, WS
Published in: J Biomed Opt
May 2015

We demonstrate a multimodal approach that combines a pump-probe with confocal reflectance and multiphoton autofluorescence microscopy. Pump-probe microscopy has been proven to be of great value in analyzing thin tissue sections of pigmented lesions, as it produces molecular contrast which is inaccessible by other means. However, the higher optical intensity required to overcome scattering in thick tissue leads to higher-order nonlinearities in the optical response of melanin (e.g., two-photon pump and one-photon probe) that present additional challenges for interpreting the data. We show that analysis of pigment composition in vivo must carefully account for signal terms that are nonlinear with respect to the pump and probe intensities. We find that pump-probe imaging gives useful contrast for pigmented structures over a large range of spatial scales (100 μm to 1 cm), making it a potentially useful tool for tracking the progression of pigmented lesions without the need to introduce exogenous contrast agents.

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Published In

J Biomed Opt

DOI

EISSN

1560-2281

Publication Date

May 2015

Volume

20

Issue

5

Start / End Page

051012

Location

United States

Related Subject Headings

  • Skin Pigmentation
  • Skin Neoplasms
  • Skin
  • Photons
  • Optics
  • Neoplasm Transplantation
  • Microscopy, Fluorescence, Multiphoton
  • Microscopy
  • Mice
  • Melanoma
 

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Wilson, J. W., Degan, S., Gainey, C. S., Mitropoulos, T., Simpson, M. J., Zhang, J. Y., & Warren, W. S. (2015). Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions. J Biomed Opt, 20(5), 051012. https://doi.org/10.1117/1.JBO.20.5.051012
Wilson, Jesse W., Simone Degan, Christina S. Gainey, Tanya Mitropoulos, Mary Jane Simpson, Jennifer Y. Zhang, and Warren S. Warren. “Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions.J Biomed Opt 20, no. 5 (May 2015): 051012. https://doi.org/10.1117/1.JBO.20.5.051012.
Wilson JW, Degan S, Gainey CS, Mitropoulos T, Simpson MJ, Zhang JY, et al. Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions. J Biomed Opt. 2015 May;20(5):051012.
Wilson, Jesse W., et al. “Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions.J Biomed Opt, vol. 20, no. 5, May 2015, p. 051012. Pubmed, doi:10.1117/1.JBO.20.5.051012.
Wilson JW, Degan S, Gainey CS, Mitropoulos T, Simpson MJ, Zhang JY, Warren WS. Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions. J Biomed Opt. 2015 May;20(5):051012.

Published In

J Biomed Opt

DOI

EISSN

1560-2281

Publication Date

May 2015

Volume

20

Issue

5

Start / End Page

051012

Location

United States

Related Subject Headings

  • Skin Pigmentation
  • Skin Neoplasms
  • Skin
  • Photons
  • Optics
  • Neoplasm Transplantation
  • Microscopy, Fluorescence, Multiphoton
  • Microscopy
  • Mice
  • Melanoma