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Disparate results between proliferation rates of surgically excised prostate tumors and an in vitro bioassay using sera from a positive randomized controlled trial.

Publication ,  Journal Article
Azrad, M; Vollmer, RT; Madden, J; Polascik, TJ; Snyder, DC; Ruffin, MT; Moul, JW; Brenner, D; He, X; Demark-Wahnefried, W
Published in: Biotech Histochem
April 2015

In vitro bioassay has been used extensively to test the effects of culturing cancer cells in sera from humans participating in dietary interventions, i.e, studies of modified intake of nutrients for the purpose of reducing cancer risk or progression. It has been hypothesized that cell proliferation rates determined by the in vitro bioassay indicate whether modification of dietary intake could decrease cancer cell growth in vivo. It has been suggested, however, that the in vitro bioassay may not correlate with tumor cell proliferation rates in prostate cancer. We investigated the concordance of cell proliferation rates from surgically excised prostate tumor tissue with the in vitro bioassay using sera from matched patients. We used samples from an earlier randomized clinical trial that showed that supplementation with flaxseed significantly inhibited prostate cancer cell proliferation rates in vivo as indicated by Ki67 staining in tumor specimens. Proliferation rates of LNCaP, DU145 and PC3 cell lines cultured in 10% human sera from participants in the flaxseed trial were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Spearman's Rho correlation coefficients (ρ) indicated no association between Ki67 staining in prostate tumors and the in vitro bioassay for the three cell lines. These disparate findings suggest that the in vitro bioassay may not provide an accurate assessment of the environment in vivo.

Duke Scholars

Published In

Biotech Histochem

DOI

EISSN

1473-7760

Publication Date

April 2015

Volume

90

Issue

3

Start / End Page

184 / 189

Location

England

Related Subject Headings

  • Seeds
  • Prostatic Neoplasms
  • Prostatectomy
  • Pathology
  • Middle Aged
  • Male
  • Ki-67 Antigen
  • Humans
  • Flax
  • Dietary Supplements
 

Citation

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ICMJE
MLA
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Azrad, M., Vollmer, R. T., Madden, J., Polascik, T. J., Snyder, D. C., Ruffin, M. T., … Demark-Wahnefried, W. (2015). Disparate results between proliferation rates of surgically excised prostate tumors and an in vitro bioassay using sera from a positive randomized controlled trial. Biotech Histochem, 90(3), 184–189. https://doi.org/10.3109/10520295.2014.976840
Azrad, M., R. T. Vollmer, J. Madden, T. J. Polascik, D. C. Snyder, M. T. Ruffin, J. W. Moul, D. Brenner, X. He, and W. Demark-Wahnefried. “Disparate results between proliferation rates of surgically excised prostate tumors and an in vitro bioassay using sera from a positive randomized controlled trial.Biotech Histochem 90, no. 3 (April 2015): 184–89. https://doi.org/10.3109/10520295.2014.976840.
Azrad M, Vollmer RT, Madden J, Polascik TJ, Snyder DC, Ruffin MT, et al. Disparate results between proliferation rates of surgically excised prostate tumors and an in vitro bioassay using sera from a positive randomized controlled trial. Biotech Histochem. 2015 Apr;90(3):184–9.
Azrad, M., et al. “Disparate results between proliferation rates of surgically excised prostate tumors and an in vitro bioassay using sera from a positive randomized controlled trial.Biotech Histochem, vol. 90, no. 3, Apr. 2015, pp. 184–89. Pubmed, doi:10.3109/10520295.2014.976840.
Azrad M, Vollmer RT, Madden J, Polascik TJ, Snyder DC, Ruffin MT, Moul JW, Brenner D, He X, Demark-Wahnefried W. Disparate results between proliferation rates of surgically excised prostate tumors and an in vitro bioassay using sera from a positive randomized controlled trial. Biotech Histochem. 2015 Apr;90(3):184–189.

Published In

Biotech Histochem

DOI

EISSN

1473-7760

Publication Date

April 2015

Volume

90

Issue

3

Start / End Page

184 / 189

Location

England

Related Subject Headings

  • Seeds
  • Prostatic Neoplasms
  • Prostatectomy
  • Pathology
  • Middle Aged
  • Male
  • Ki-67 Antigen
  • Humans
  • Flax
  • Dietary Supplements