Skip to main content

Genome-wide chromatin footprinting reveals changes in replication origin architecture induced by pre-RC assembly.

Publication ,  Journal Article
Belsky, JA; MacAlpine, HK; Lubelsky, Y; Hartemink, AJ; MacAlpine, DM
Published in: Genes Dev
January 15, 2015

Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replication origins. To reveal how the chromatin architecture modulates origin selection and activation, we "footprinted" nucleosomes, transcription factors, and replication proteins at multiple points during the Saccharomyces cerevisiae cell cycle. Our nucleotide-resolution protein occupancy profiles resolved a precise ORC-dependent footprint at 269 origins in G2. A separate class of inefficient origins exhibited protein occupancy only in G1, suggesting that stable ORC chromatin association in G2 is a determinant of origin efficiency. G1 nucleosome remodeling concomitant with pre-RC assembly expanded the origin nucleosome-free region and enhanced activation efficiency. Finally, the local chromatin environment restricts the loading of the Mcm2-7 double hexamer either upstream of or downstream from the ARS consensus sequence (ACS).

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genes Dev

DOI

EISSN

1549-5477

Publication Date

January 15, 2015

Volume

29

Issue

2

Start / End Page

212 / 224

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Origin Recognition Complex
  • Nucleosomes
  • Minichromosome Maintenance Proteins
  • Genome-Wide Association Study
  • G2 Phase
  • G1 Phase
  • Developmental Biology
  • Chromatin
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Belsky, J. A., MacAlpine, H. K., Lubelsky, Y., Hartemink, A. J., & MacAlpine, D. M. (2015). Genome-wide chromatin footprinting reveals changes in replication origin architecture induced by pre-RC assembly. Genes Dev, 29(2), 212–224. https://doi.org/10.1101/gad.247924.114
Belsky, Jason A., Heather K. MacAlpine, Yoav Lubelsky, Alexander J. Hartemink, and David M. MacAlpine. “Genome-wide chromatin footprinting reveals changes in replication origin architecture induced by pre-RC assembly.Genes Dev 29, no. 2 (January 15, 2015): 212–24. https://doi.org/10.1101/gad.247924.114.
Belsky JA, MacAlpine HK, Lubelsky Y, Hartemink AJ, MacAlpine DM. Genome-wide chromatin footprinting reveals changes in replication origin architecture induced by pre-RC assembly. Genes Dev. 2015 Jan 15;29(2):212–24.
Belsky, Jason A., et al. “Genome-wide chromatin footprinting reveals changes in replication origin architecture induced by pre-RC assembly.Genes Dev, vol. 29, no. 2, Jan. 2015, pp. 212–24. Pubmed, doi:10.1101/gad.247924.114.
Belsky JA, MacAlpine HK, Lubelsky Y, Hartemink AJ, MacAlpine DM. Genome-wide chromatin footprinting reveals changes in replication origin architecture induced by pre-RC assembly. Genes Dev. 2015 Jan 15;29(2):212–224.

Published In

Genes Dev

DOI

EISSN

1549-5477

Publication Date

January 15, 2015

Volume

29

Issue

2

Start / End Page

212 / 224

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Origin Recognition Complex
  • Nucleosomes
  • Minichromosome Maintenance Proteins
  • Genome-Wide Association Study
  • G2 Phase
  • G1 Phase
  • Developmental Biology
  • Chromatin