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MiR-21 alleviates secondary blood-brain barrier damage after traumatic brain injury in rats.

Publication ,  Journal Article
Ge, X; Han, Z; Chen, F; Wang, H; Zhang, B; Jiang, R; Lei, P; Zhang, J
Published in: Brain Res
April 7, 2015

Our recent studies have identified increased expression of miR-21 in brain following traumatic brain injury (TBI), which alleviated brain edema that related to the blood-brain barrier (BBB) leakage. To analyze the potential effect of miR-21 on secondary BBB damage after TBI, we employed the fluid percussion injury rat model and manipulated the expression level of miR-21 in brain. We found that miR-21 level in brain microvascular endothelial cells (BMVECs) in lesioned cerebral cortex can be upregulated or downregulated by intracerebroventricular infusion of miR-21 agomir or antagomir. Upregulated miR-21 level conferred a better neurological outcome of TBI, and alleviated TBI-induced secondary BBB damage and loss of tight junction proteins. To explore the molecular mechanism underlying this protective effect, we detected the impact of miR-21 on the expression of Angiopoietin-1(Ang-1) and Tie-2, which can promote the expression of tight junction proteins and amplify BBB stabilization. We found that miR-21 exerts the protective effect on BBB by activating the Ang-1/Tie-2 axis in BMVECs. Thus, miR-21 could be a potential therapeutic target for interventions of secondary BBB damage after TBI.

Duke Scholars

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Published In

Brain Res

DOI

EISSN

1872-6240

Publication Date

April 7, 2015

Volume

1603

Start / End Page

150 / 157

Location

Netherlands

Related Subject Headings

  • Rats, Sprague-Dawley
  • Random Allocation
  • RNA, Messenger
  • Occludin
  • Neurology & Neurosurgery
  • Microvessels
  • MicroRNAs
  • Male
  • Endothelial Cells
  • Disease Models, Animal
 

Citation

APA
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ICMJE
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Ge, X., Han, Z., Chen, F., Wang, H., Zhang, B., Jiang, R., … Zhang, J. (2015). MiR-21 alleviates secondary blood-brain barrier damage after traumatic brain injury in rats. Brain Res, 1603, 150–157. https://doi.org/10.1016/j.brainres.2015.01.009
Ge, Xintong, Zhaoli Han, Fanglian Chen, Haichen Wang, Baoliang Zhang, Rongcai Jiang, Ping Lei, and Jianning Zhang. “MiR-21 alleviates secondary blood-brain barrier damage after traumatic brain injury in rats.Brain Res 1603 (April 7, 2015): 150–57. https://doi.org/10.1016/j.brainres.2015.01.009.
Ge X, Han Z, Chen F, Wang H, Zhang B, Jiang R, et al. MiR-21 alleviates secondary blood-brain barrier damage after traumatic brain injury in rats. Brain Res. 2015 Apr 7;1603:150–7.
Ge, Xintong, et al. “MiR-21 alleviates secondary blood-brain barrier damage after traumatic brain injury in rats.Brain Res, vol. 1603, Apr. 2015, pp. 150–57. Pubmed, doi:10.1016/j.brainres.2015.01.009.
Ge X, Han Z, Chen F, Wang H, Zhang B, Jiang R, Lei P, Zhang J. MiR-21 alleviates secondary blood-brain barrier damage after traumatic brain injury in rats. Brain Res. 2015 Apr 7;1603:150–157.
Journal cover image

Published In

Brain Res

DOI

EISSN

1872-6240

Publication Date

April 7, 2015

Volume

1603

Start / End Page

150 / 157

Location

Netherlands

Related Subject Headings

  • Rats, Sprague-Dawley
  • Random Allocation
  • RNA, Messenger
  • Occludin
  • Neurology & Neurosurgery
  • Microvessels
  • MicroRNAs
  • Male
  • Endothelial Cells
  • Disease Models, Animal