Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering.

Published

Journal Article

Since bone repair and regeneration depend on vasculogenesis and osteogenesis, both of these processes are essential for successful vascularized bone engineering. Using adipose-derived stem cells (ASCs), we investigated temporal gene expression profiles, as well as bone nodule and endothelial tubule formation capacities, during osteogenic and vasculogenic ASC lineage commitment. Osteoprogenitor-enriched cell populations were found to express RUNX2, MSX2, SP7 (osterix), BGLAP (osteocalcin), SPARC (osteonectin), and SPP1 (osteopontin) in a temporally specific sequence. Irreversible commitment of ASCs to the osteogenic lineage occurred between days 6 and 9 of differentiation. Endothelioprogenitor-enriched cell populations expressed CD34, PECAM1 (CD31), ENG (CD105), FLT1 (Vascular endothelial growth factor [VEGFR1]), and KDR (VEGFR2). Capacity for microtubule formation was evident in as early as 3 days. Functional capacity was assessed in eight coculture combinations for both bone nodule and endothelial tubule formation, and the greatest expression of these end-differentiation phenotypes was observed in the combination of well-differentiated endothelial cells with less-differentiated osteoblastic cells. Taken together, our results demonstrate vascularized bone engineering utilizing ASCs is a promising enterprise, and that coculture strategies should focus on developing a more mature vascular network in combination with a less mature osteoblastic stromal cell.

Full Text

Duke Authors

Cited Authors

  • Valenzuela, CD; Allori, AC; Reformat, DD; Sailon, AM; Allen, RJ; Davidson, EH; Alikhani, M; Bromage, TG; Ricci, JL; Warren, SM

Published Date

  • June 2013

Published In

Volume / Issue

  • 19 / 11-12

Start / End Page

  • 1373 - 1385

PubMed ID

  • 23343199

Pubmed Central ID

  • 23343199

Electronic International Standard Serial Number (EISSN)

  • 1937-335X

Digital Object Identifier (DOI)

  • 10.1089/ten.TEA.2012.0323

Language

  • eng

Conference Location

  • United States