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Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering.

Publication ,  Journal Article
Valenzuela, CD; Allori, AC; Reformat, DD; Sailon, AM; Allen, RJ; Davidson, EH; Alikhani, M; Bromage, TG; Ricci, JL; Warren, SM
Published in: Tissue Eng Part A
June 2013

Since bone repair and regeneration depend on vasculogenesis and osteogenesis, both of these processes are essential for successful vascularized bone engineering. Using adipose-derived stem cells (ASCs), we investigated temporal gene expression profiles, as well as bone nodule and endothelial tubule formation capacities, during osteogenic and vasculogenic ASC lineage commitment. Osteoprogenitor-enriched cell populations were found to express RUNX2, MSX2, SP7 (osterix), BGLAP (osteocalcin), SPARC (osteonectin), and SPP1 (osteopontin) in a temporally specific sequence. Irreversible commitment of ASCs to the osteogenic lineage occurred between days 6 and 9 of differentiation. Endothelioprogenitor-enriched cell populations expressed CD34, PECAM1 (CD31), ENG (CD105), FLT1 (Vascular endothelial growth factor [VEGFR1]), and KDR (VEGFR2). Capacity for microtubule formation was evident in as early as 3 days. Functional capacity was assessed in eight coculture combinations for both bone nodule and endothelial tubule formation, and the greatest expression of these end-differentiation phenotypes was observed in the combination of well-differentiated endothelial cells with less-differentiated osteoblastic cells. Taken together, our results demonstrate vascularized bone engineering utilizing ASCs is a promising enterprise, and that coculture strategies should focus on developing a more mature vascular network in combination with a less mature osteoblastic stromal cell.

Duke Scholars

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

June 2013

Volume

19

Issue

11-12

Start / End Page

1373 / 1385

Location

United States

Related Subject Headings

  • Young Adult
  • Tissue Engineering
  • Osteogenesis
  • Osteocytes
  • Neovascularization, Physiologic
  • Middle Aged
  • Microtubules
  • Mesenchymal Stem Cells
  • Humans
  • Human Umbilical Vein Endothelial Cells
 

Citation

APA
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MLA
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Valenzuela, C. D., Allori, A. C., Reformat, D. D., Sailon, A. M., Allen, R. J., Davidson, E. H., … Warren, S. M. (2013). Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering. Tissue Eng Part A, 19(11–12), 1373–1385. https://doi.org/10.1089/ten.TEA.2012.0323
Valenzuela, Cristian D., Alexander C. Allori, Derek D. Reformat, Alexander M. Sailon, Robert J. Allen, Edward H. Davidson, Mani Alikhani, Timothy G. Bromage, John L. Ricci, and Stephen M. Warren. “Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering.Tissue Eng Part A 19, no. 11–12 (June 2013): 1373–85. https://doi.org/10.1089/ten.TEA.2012.0323.
Valenzuela CD, Allori AC, Reformat DD, Sailon AM, Allen RJ, Davidson EH, et al. Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering. Tissue Eng Part A. 2013 Jun;19(11–12):1373–85.
Valenzuela, Cristian D., et al. “Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering.Tissue Eng Part A, vol. 19, no. 11–12, June 2013, pp. 1373–85. Pubmed, doi:10.1089/ten.TEA.2012.0323.
Valenzuela CD, Allori AC, Reformat DD, Sailon AM, Allen RJ, Davidson EH, Alikhani M, Bromage TG, Ricci JL, Warren SM. Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering. Tissue Eng Part A. 2013 Jun;19(11–12):1373–1385.

Published In

Tissue Eng Part A

DOI

EISSN

1937-335X

Publication Date

June 2013

Volume

19

Issue

11-12

Start / End Page

1373 / 1385

Location

United States

Related Subject Headings

  • Young Adult
  • Tissue Engineering
  • Osteogenesis
  • Osteocytes
  • Neovascularization, Physiologic
  • Middle Aged
  • Microtubules
  • Mesenchymal Stem Cells
  • Humans
  • Human Umbilical Vein Endothelial Cells