Mutant IDH is sufficient to initiate enchondromatosis in mice.

Published

Journal Article

Enchondromas are benign cartilage tumors and precursors to malignant chondrosarcomas. Somatic mutations in the isocitrate dehydrogenase genes (IDH1 and IDH2) are present in the majority of these tumor types. How these mutations cause enchondromas is unclear. Here, we identified the spectrum of IDH mutations in human enchondromas and chondrosarcomas and studied their effects in mice. A broad range of mutations was identified, including the previously unreported IDH1-R132Q mutation. These mutations harbored enzymatic activity to catalyze α-ketoglutarate to d-2-hydroxyglutarate (d-2HG). Mice expressing Idh1-R132Q in one allele in cells expressing type 2 collagen showed a disordered growth plate, with persistence of type X-expressing chondrocytes. Chondrocyte cell cultures from these animals or controls showed that there was an increase in proliferation and expression of genes characteristic of hypertrophic chondrocytes with expression of Idh1-R132Q or 2HG treatment. Col2a1-Cre;Idh1-R132Q mutant knock-in mice (mutant allele expressed in chondrocytes) did not survive after the neonatal stage. Col2a1-Cre/ERT2;Idh1-R132 mutant conditional knock-in mice, in which Cre was induced by tamoxifen after weaning, developed multiple enchondroma-like lesions. Taken together, these data show that mutant IDH or d-2HG causes persistence of chondrocytes, giving rise to rests of growth-plate cells that persist in the bone as enchondromas.

Full Text

Duke Authors

Cited Authors

  • Hirata, M; Sasaki, M; Cairns, RA; Inoue, S; Puviindran, V; Li, WY; Snow, BE; Jones, LD; Wei, Q; Sato, S; Tang, YJ; Nadesan, P; Rockel, J; Whetstone, H; Poon, R; Weng, A; Gross, S; Straley, K; Gliser, C; Xu, Y; Wunder, J; Mak, TW; Alman, BA

Published Date

  • March 2015

Published In

Volume / Issue

  • 112 / 9

Start / End Page

  • 2829 - 2834

PubMed ID

  • 25730874

Pubmed Central ID

  • 25730874

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1424400112

Language

  • eng