Liposome reconstitution and transport assay for recombinant transporters.

Journal Article

Secondary active transporters are responsible for the cellular uptake of many biologically important molecules, including neurotransmitters, nutrients, and drugs. Because of their physiological and clinical importance, a method for assessing their transport activity in vitro is necessary to gain a better understanding of how these transporters function at the molecular level. In this chapter, we describe a protocol for reconstituting the concentrative nucleoside transporter from Vibrio cholerae into proteoliposomes. We then describe a radiolabeled substrate uptake assay that can be used to functionally characterize the transporter. These methods are relatively common and can be applied to other secondary active transporters, with or without some modification.

Full Text

Duke Authors

Cited Authors

  • Johnson, ZL; Lee, S-Y

Published Date

  • January 2015

Published In

Volume / Issue

  • 556 /

Start / End Page

  • 373 - 383

PubMed ID

  • 25857791

Electronic International Standard Serial Number (EISSN)

  • 1557-7988

International Standard Serial Number (ISSN)

  • 0076-6879

Digital Object Identifier (DOI)

  • 10.1016/bs.mie.2014.11.048

Language

  • eng