Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • Ph.D., University of California at Berkeley 2003
  • B.S., Yonsei University 1998
• 

  Duke Appointment History

  • Assistant Professor of Biochemistry, Biochemistry, Basic Science Departments 2009 - 2016
• Recognition
• 

  In the News

  • APR 17, 2017

    Time-Lapse Shows How Anticancer and Antiviral Drugs Get Into Cells

  • JAN 15, 2016

    Scientists Discover Blueprint of Body's Temperature Sensor
• 

  Awards & Honors

  • NIGMS award, 56th Biophysical Society Annual Meeting. NIGMS. 2012
  • Basil O'connor Starter Scholar Research Award. March of Dimes Foundation. 2011
  • NIH Director’s New Innovator Award. NIH. 2011
  • Sloan Research Fellowship-Neuroscience. Alfred P. Sloan Foundation. 2011
  • Klingenstein Fellowship Award in the Neurosciences. Klingenstein Foundation. 2010
  • Mallinckrodt Scholar Award. Mallinckrodt Foundation. 2010
  • McKnight Scholar Award. McKnight Foundation. 2010
• Research
• 

  Selected Grants

  • Structure, function, and pharmacology of neuronal membrane transport proteins awarded by National Institutes of Health 2016 - 2024
  • Structural and Mechanistic Characterization of MraY Catalysis and Inhibition awarded by National Institutes of Health 2017 - 2020
  • Development of novel therapeutics for pain and itch relief awarded by National Institutes of Health 2014 - 2018
  • Structure and Function of Concentrative Nucleoside Transporters awarded by National Institutes of Health 2013 - 2017
  • Pharmacology and biophysics of the voltage-gated sodium channel Nav1.7: a therapeutic target for pain awarded by National Institutes of Health 2011 - 2016
  • Structural Biology and Biophysics Training Program awarded by National Institutes of Health 1994 - 2015
  • High sensitivity multi-purpose electron paramagnetic resonance spectroscopy for biotechnological and biomedical research awarded by North Carolina Biotechnology Center 2014 - 2015
  • Automated detection of protein crystals in high-throughput crystallography experiments awarded by North Carolina Biotechnology Center 2014 - 2015
  • New Console and Cold Probe for the Duke 600 MHz NMR Spectrometer System awarded by National Institutes of Health 2013 - 2014
• Publications & Artistic Works
• 

  Selected Publications

  • Journal Articles

    • Yoo J, Wu M, Yin Y, Herzik MA, Lander GC, Lee S-Y. Cryo-EM structure of a mitochondrial calcium uniporter. Science (New York, N.Y.). 2018 Aug;361(6401):506-511. PubMed Central PMCID: 29954988.  Full Text
    • Zubcevic L, Le S, Yang H, Lee S-Y. Conformational plasticity in the selectivity filter of the TRPV2 ion channel. Nature Structural & Molecular Biology. 2018 May;25(5):405-415.  Full Text
    • Yoo J, Mashalidis EH, Kuk ACY, Yamamoto K, Kaeser B, Ichikawa S, Lee S-Y. GlcNAc-1-P-transferase–tunicamycin complex structure reveals basis for inhibition of N-glycosylation. Nature Structural & Molecular Biology. 2018 Mar;25(3):217-224.  Full Text
    • Yoo J, Mashalidis EH, Kuk ACY, Yamamoto K, Kaeser B, Ichikawa S, Lee S-Y. GlcNAc-1-P-transferase–tunicamycin complex structure reveals basis for inhibition of N-glycosylation. Nature Structural & Molecular Biology. 2018 Mar;25(3):217-224.  Full Text
    • Bang S, Yoo J, Gong X, Liu D, Han Q, Luo X, Chang W, Chen G, Im S-T, Kim YH, Strong JA, Zhang M-Z, Zhang J-M, Lee S-Y, Ji R-R. Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant Monoclonal Antibodies that Target Nav1.7 : Differential activities of Nav1.7-targeting monoclonal antibodies. Neuroscience Bulletin. 2018 Feb;34(1):22-41. PubMed Central PMCID: 29333591.  Full Text
    • Chang W, Berta T, Kim YH, Lee S, Lee S-Y, Ji R-R. Expression and Role of Voltage-Gated Sodium Channels in Human Dorsal Root Ganglion Neurons with Special Focus on Nav1.7, Species Differences, and Regulation by Paclitaxel. Neuroscience Bulletin. 2018 Feb;34(1):4-12. PubMed Central PMCID: 28424991.  Full Text
    • Yin Y, Wu M, Zubcevic L, Borschel WF, Lander GC, Lee SY. Structure of the cold- And menthol-sensing ion channel TRPM8. Science. 2018 Jan 12;359(6372):237-241.  Full Text  Open Access Copy  Link to Item
    • Hirschi M, Herzik MA, Wie J, Suo Y, Borschel WF, Ren D, Lander GC, Lee S-Y. Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3. Nature. 2017 Oct 11;550(7676):411-414. PubMed Central PMCID: 29019979.  Full Text
    • Hirschi M, Johnson ZL, Lee S-Y. Visualizing multistep elevator-like transitions of a nucleoside transporter. Nature. 2017 May;545(7652):66-70. PubMed Central PMCID: 28424521.  Full Text
    • Kuk ACY, Mashalidis EH, Lee S-Y. Crystal structure of the MOP flippase MurJ in an inward-facing conformation. Nature Structural & Molecular Biology. 2017 Feb;24(2):171-176. PubMed Central PMCID: 28024149.  Full Text
    • Pitt GS, Lee S-Y. Current view on regulation of voltage-gated sodium channels by calcium and auxiliary proteins. Protein science : a publication of the Protein Society. 2016 Sep;25(9):1573-1584. PubMed PMID: 27262167. (Review)  Full Text
    • Chung BC, Mashalidis EH, Tanino T, Kim M, Matsuda A, Hong J, Ichikawa S, Lee S-Y. Structural insights into inhibition of lipid I production in bacterial cell wall synthesis. Nature. 2016 May;533(7604):557-560. PubMed Central PMCID: 27088606.  Full Text
    • Zubcevic L, Herzik MA, Chung BC, Liu Z, Lander GC, Lee S-Y. Cryo-electron microscopy structure of the TRPV2 ion channel. Nature Structural & Molecular Biology. 2016 Feb;23(2):180-186. PubMed Central PMCID: 26779611.  Full Text
    • Johnson ZL, Lee S-Y. Liposome reconstitution and transport assay for recombinant transporters. Methods in enzymology. 2015 Jan;556:373-383. PubMed PMID: 25857791.  Full Text
    • Wang C, Chung BC, Yan H, Wang H-G, Lee S-Y, Pitt GS. Structural analyses of Ca²⁺/CaM interaction with NaV channel C-termini reveal mechanisms of calcium-dependent regulation. Nature Communications. 2014 Sep 18;5:4896-null. PubMed Central PMCID: 25232683.  Full Text
    • Johnson ZL, Lee J-H, Lee K, Lee M, Kwon D-Y, Hong J, Lee S-Y. Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters. Elife. 2014 Jul 31;3:e03604-null. PubMed Central PMCID: 25082345.  Full Text
    • Johnson ZL, Lee J-H, Lee K, Lee M, Kwon D-Y, Hong J, Lee S-Y. Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters. ELIFE. 2014 Jul 31;3.  Full Text  Link to Item
    • Lee J-H, Park C-K, Chen G, Han Q, Xie R-G, Liu T, Ji R-R, Lee S-Y. A monoclonal antibody that targets a NaV1.7 channel voltage sensor for pain and itch relief. Cell. 2014 Jun;157(6):1393-1404. PubMed Central PMCID: 24856969.  Full Text
    • Chung BC, Zhao J, Gillespie RA, Kwon D-Y, Guan Z, Hong J, Zhou P, Lee S-Y. Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis. Science (New York, N.Y.). 2013 Aug;341(6149):1012-1016. PubMed Central PMCID: 23990562.  Full Text
    • Chung BC, Zhao J, Gillespie RA, Kwon D-Y, Guan Z, Hong J, Zhou P, Lee S-Y. Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis. Science. 2013;341(6149):1012-1016.  Full Text
    • Wang C, Chung BC, Yan H, Lee S-Y, Pitt GS. Crystal structure of the ternary complex of a NaV C-terminal domain, a fibroblast growth factor homologous factor, and calmodulin. Structure. 2012 Jul 3;20(7):1167-1176. PubMed PMID: 22705208.  Full Text  Link to Item
    • Johnson ZL, Cheong C-G, Lee S-Y. Crystal structure of a concentrative nucleoside transporter from Vibrio cholerae at 2.4 Å. Nature. 2012 Mar 11;483(7390):489-493. PubMed Central PMCID: 22407322.  Full Text
    • Lee S-Y, Letts JA, MacKinnon R. Functional reconstitution of purified human Hv1 H+ channels. Journal of Molecular Biology. 2009 Apr;387(5):1055-1060. PubMed Central PMCID: 19233200.  Full Text
    • Lee S-Y, Banerjee A, MacKinnon R. Two Separate Interfaces between the Voltage Sensor and Pore Are Required for the Function of Voltage-Dependent K ⁺ Channels. PLoS Biology. 2009;7(3). PubMed PMID: 19260762.  Full Text
    • Lee S-Y, Banerjee A, Mackinnon R. Two separate interfaces between the Voltage sensor and pore are required for the function of Voltage-dependent K⁺ channels. PLoS Biology. 2009;7(3):0676-0686.  Full Text
    • Lee S-Y, Letts JA, Mackinnon R. Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1. Proceedings of the National Academy of Sciences of the United States of America. 2008 Jun;105(22):7692-7695. PubMed Central PMCID: 18509058.  Full Text
    • Kim HS, Damo SM, Lee S-Y, Wemmer D, Klinman JP. Structure and hydride transfer mechanism of a moderate thermophilic dihydrofolate reductase from Bacillus stearothermophilus and comparison to its mesophilic and hyperthermophilic homologues. Biochemistry. 2005;44(34):11428-11439. PubMed PMID: 16114879.  Full Text
    • Lee S-Y, Lee A, Chen J, MacKinnon R. Structure of the KvAP voltage-dependent K⁺ channel and its on the lipid membrane. Proceedings of the National Academy of Sciences of the United States of America. 2005;102(43):15441-15446. PubMed PMID: 16223877.  Full Text
    • Roberts A, Lee S-Y, McCullagh E, Silversmith RE, Wemmer DE. YbiV from Escherichia coli K12 is a HAD phosphatase. Proteins: Structure, Function and Genetics. 2005;58(4):790-801. PubMed PMID: 15657928.  Full Text
    • Lee S-Y, MacKinnon R. A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom. Nature. 2004 Jul;430(6996):232-235. PubMed Central PMCID: 15241419.  Full Text
    • Gardino AK, Volkman BF, Cho HS, Lee S-Y, Wemmer DE, Kern D. The NMR solution structure of BeF₃^--activated Spo0F reveals the conformational switch in a phosphorelay system. Journal of Molecular Biology. 2003;331(1):245-254. PubMed PMID: 12875849.  Full Text
    • Hastings CA, Lee S-Y, Cho HS, Yan D, Kustu S, Wemmer DE. High-resolution solution structure of the beryllofluoride-activated NtrC receiver domain. Biochemistry. 2003;42(30):9081-9090. PubMed PMID: 12885241.  Full Text
    • Lee S-Y, Torre ADL, Yan D, Kustu S, Nixon BT, Wemmer DE. Regulation of the transcriptional activator NtrC1: Structural studies of the regulatory and AAA⁺ ATPase domains. Genes and Development. 2003;17(20):2552-2563. PubMed PMID: 14561776.  Full Text
    • Rubin SM, Lee S-Y, Ruiz EJ, Pines A, Wemmer DE. Detection and characterization of xenon-binding sites in proteins by ¹²⁹Xe NMR spectroscopy. Journal of Molecular Biology. 2002;322(2):425-440. PubMed PMID: 12217701.  Full Text
    • Lee SY, Cho HS, Pelton JG, Yan D, Berry EA, Wemmer DE. Crystal structure of activated CheY. Comparison with other activated receiver domains. The Journal of Biological Chemistry. 2001 May;276(19):16425-16431. PubMed Central PMCID: 11279165.  Full Text
    • Lee SY, Cho HS, Pelton JG, Yan D, Henderson RK, King DS, Huang L, Kustu S, Berry EA, Wemmer DE. Crystal structure of an activated response regulator bound to its target. Nature Structural Biology. 2001 Jan;8(1):52-56. PubMed Central PMCID: 11135671.  Full Text
    • Cho HS, Lee S-Y, Yan D, Pan X, Parkinson JS, Kustu S, Wemmer DE, Pelton JG. NMR structure of activated CheY. Journal of Molecular Biology. 2000;297(3):543-551. PubMed PMID: 10731410.  Full Text
    • Lee S-Y, Lee J-H, Chang H-J, Cho JM, Jung J-W, Lee W. Solution structure of a sweet protein single-chain monellin determined by nuclear magnetic resonance and dynamical simulated annealing calculations. Biochemistry. 1999;38(8):2340-2346. PubMed PMID: 10029527.  Full Text
    • Yan D, Cho HS, Hastings CA, Igo MM, Lee S-Y, Pelton JG, Stewart V, Wemmer DE, Kustu S. Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators. Proceedings of the National Academy of Sciences of the United States of America. 1999;96(26):14789-14794. PubMed PMID: 10611291.  Full Text
    • Li HL, Sui HX, Ghanshani S, Lee S, Walian PJ, Wu CL, Chandy KG, Jap BK. Two-dimensional crystallization and projection structure of KcsA potassium channel. Journal of molecular biology. 1998 Sep;282(2):211-216. PubMed PMID: 9735281.  Full Text
• Teaching & Mentoring
• 

  Recent Courses

  • BIOCHEM 393: Research Independent Study 2018
  • BIOCHEM 593: Research Independent Study 2018

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