Scholars@Duke

• Background
• 

  Education, Training, & Certifications

  • Ph.D., University of California - Berkeley 2003
  • B.S., Yonsei University (South Korea) 1998
• 

  Previous Appointments & Affiliations

  • Associate Professor of Biochemistry, Biochemistry, Basic Science Departments 2016 - 2020
  • Assistant Professor of Biochemistry, Biochemistry, Basic Science Departments 2009 - 2016
• Recognition
• 

  In the News

  • DEC 19, 2019

    Cryo-Electron Microscope Captures Details of the ‘Wasabi Sensor’

  • FEB 7, 2019

    Defining the Shape of Cool

  • APR 17, 2017

    Time-Lapse Shows How Anticancer and Antiviral Drugs Get Into Cells

  • JAN 15, 2016

    Scientists Discover Blueprint of Body's Temperature Sensor
• 

  Awards & Honors

  • NIGMS award, 56th Biophysical Society Annual Meeting. NIGMS. 2012
  • Basil O'connor Starter Scholar Research Award. March of Dimes Foundation. 2011
  • NIH Director’s New Innovator Award. NIH. 2011
  • Sloan Research Fellowship-Neuroscience. Alfred P. Sloan Foundation. 2011
  • Klingenstein Fellowship Award in the Neurosciences. Klingenstein Foundation. 2010
  • Mallinckrodt Scholar Award. Mallinckrodt Foundation. 2010
  • McKnight Scholar Award. McKnight Foundation. 2010
• Research
• 

  Selected Grants

  • Catalysis and inhibition of chitin synthesis from pathogenic fungi awarded by National Institutes of Health 2022 - 2027
  • Cell and Molecular Biology Training Program awarded by National Institutes of Health 2021 - 2026
  • Structural and physiological studies on the human equilibrative nucleoside transporter 3 awarded by Helen Hay Whitney Foundation 2022 - 2025
  • Pharmacological Sciences Training Grant awarded by National Institutes of Health 2020 - 2025
  • Structure, function, and pharmacology of neuronal membrane transport proteins awarded by National Institutes of Health 2016 - 2024
  • TRPM8 in eye health and disease awarded by National Institutes of Health 2020 - 2024
  • Structural and chemical biology of human equilibrative nucleoside transporters awarded by National Institutes of Health 2020 - 2024
  • Structural studies of insect TRP channels awarded by BASF Corporation 2020 - 2023
  • Structure guided design of novel adenosine reuptake inhibitors awarded by  American Heart Association 2020 - 2021
  • Structural and Mechanistic Characterization of MraY Catalysis and Inhibition awarded by National Institutes of Health 2017 - 2020
  • Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
  • Conformational ensembles associated with TRPV3 gating awarded by Scripps Research Institute 2018 - 2020
  • Development of novel therapeutics for pain and itch relief awarded by National Institutes of Health 2014 - 2018
  • Structure and Function of Concentrative Nucleoside Transporters awarded by National Institutes of Health 2013 - 2017
  • Pharmacology and biophysics of the voltage-gated sodium channel Nav1.7: a therapeutic target for pain awarded by National Institutes of Health 2011 - 2016
  • Structural Biology and Biophysics Training Program awarded by National Institutes of Health 1994 - 2015
  • High sensitivity multi-purpose electron paramagnetic resonance spectroscopy for biotechnological and biomedical research awarded by North Carolina Biotechnology Center 2014 - 2015
  • Automated detection of protein crystals in high-throughput crystallography experiments awarded by North Carolina Biotechnology Center 2014 - 2015
  • New Console and Cold Probe for the Duke 600 MHz NMR Spectrometer System awarded by National Institutes of Health 2013 - 2014
• Publications & Artistic Works
• 

  Selected Publications

  • Academic Articles

    • Kuk ACY, Hao A, Lee S-Y. Structure and Mechanism of the Lipid Flippase MurJ. Annu Rev Biochem. 2022 Jun 21;91:705–29.
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    • Wright NJ, Lee S-Y. Recent advances on the inhibition of human solute carriers: Therapeutic implications and mechanistic insights. Curr Opin Struct Biol. 2022 Jun;74:102378.
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    • Kwon DH, Zhang F, Fedor JG, Suo Y, Lee S-Y. Vanilloid-dependent TRPV1 opening trajectory from cryoEM ensemble analysis. Nat Commun. 2022 May 24;13(1):2874.
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    • Ji R-R, Lee S-Y. Molecular Sensors of Temperature, Pressure, and Pain with Special Focus on TRPV1, TRPM8, and PIEZO2 Ion Channels. Neurosci Bull. 2021 Dec;37(12):1745–9.
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    • Kwon DH, Zhang F, Suo Y, Bouvette J, Borgnia MJ, Lee S-Y. Heat-dependent opening of TRPV1 in the presence of capsaicin. Nat Struct Mol Biol. 2021 Jul;28(7):554–63.
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    • Wright NJ, Lee S-Y. Toward a Molecular Basis of Cellular Nucleoside Transport in Humans. Chem Rev. 2021 May 12;121(9):5336–58.
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    • Kwak S-H, Lim WY, Hao A, Mashalidis EH, Kwon D-Y, Jeong P, et al. Synthesis and evaluation of cyclopentane-based muraymycin analogs targeting MraY. Eur J Med Chem. 2021 Apr 5;215:113272.
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    • Yin Y, Lee S-Y. Current View of Ligand and Lipid Recognition by the Menthol Receptor TRPM8. Trends Biochem Sci. 2020 Sep;45(9):806–19.
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    • Mashalidis EH, Lee S-Y. Structures of Bacterial MraY and Human GPT Provide Insights into Rational Antibiotic Design. J Mol Biol. 2020 Aug 21;432(18):4946–63.
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    • Suo Y, Wang Z, Zubcevic L, Hsu AL, He Q, Borgnia MJ, et al. Structural Insights into Electrophile Irritant Sensing by the Human TRPA1 Channel. Neuron. 2020 Mar 4;105(5):882-894.e5.
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    • Zubcevic L, Lee S-Y. The role of π-helices in TRP channel gating. Curr Opin Struct Biol. 2019 Oct;58:314–23.
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    • Yin Y, Wu M, Hsu AL, Borschel WF, Borgnia MJ, Lander GC, et al. Visualizing structural transitions of ligand-dependent gating of the TRPM2 channel. Nat Commun. 2019 Aug 20;10(1):3740.
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    • Mashalidis EH, Kaeser B, Terasawa Y, Katsuyama A, Kwon D-Y, Lee K, et al. Chemical logic of MraY inhibition by antibacterial nucleoside natural products. Nat Commun. 2019 Jul 2;10(1):2917.
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    • Wright NJ, Lee S-Y. Structures of human ENT1 in complex with adenosine reuptake inhibitors. Nat Struct Mol Biol. 2019 Jul;26(7):599–606.
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    • Zubcevic L, Hsu AL, Borgnia MJ, Lee S-Y. Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes. Elife. 2019 May 15;8.
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    • Zubcevic L, Borschel WF, Hsu AL, Borgnia MJ, Lee S-Y. Regulatory switch at the cytoplasmic interface controls TRPV channel gating. Elife. 2019 May 9;8.
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    • Kuk ACY, Hao A, Guan Z, Lee S-Y. Visualizing conformation transitions of the Lipid II flippase MurJ. Nat Commun. 2019 Apr 15;10(1):1736.
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    • Yin Y, Le SC, Hsu AL, Borgnia MJ, Yang H, Lee S-Y. Structural basis of cooling agent and lipid sensing by the cold-activated TRPM8 channel. Science. 2019 Mar 1;363(6430).
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    • Zubcevic L, Herzik MA, Wu M, Borschel WF, Hirschi M, Song AS, et al. Conformational ensemble of the human TRPV3 ion channel. Nat Commun. 2018 Nov 14;9(1):4773.
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    • Yoo J, Wu M, Yin Y, Herzik MA, Lander GC, Lee S-Y. Cryo-EM structure of a mitochondrial calcium uniporter. Science. 2018 Aug 3;361(6401):506–11.
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    • Zubcevic L, Le S, Yang H, Lee S-Y. Conformational plasticity in the selectivity filter of the TRPV2 ion channel. Nat Struct Mol Biol. 2018 May;25(5):405–15.
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    • Yoo J, Mashalidis EH, Kuk ACY, Yamamoto K, Kaeser B, Ichikawa S, et al. GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis for inhibition of N-glycosylation. Nat Struct Mol Biol. 2018 Mar;25(3):217–24.
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    • Yoo J, Mashalidis EH, Kuk ACY, Yamamoto K, Kaeser B, Ichikawa S, et al. GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis for inhibition of N-glycosylation. Nat Struct Mol Biol. 2018 Mar;25(3):217–24.
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    • Bang S, Yoo J, Gong X, Liu D, Han Q, Luo X, et al. Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant Monoclonal Antibodies that Target Nav1.7 : Differential activities of Nav1.7-targeting monoclonal antibodies. Neurosci Bull. 2018 Feb;34(1):22–41.
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    • Chang W, Berta T, Kim YH, Lee S, Lee S-Y, Ji R-R. Expression and Role of Voltage-Gated Sodium Channels in Human Dorsal Root Ganglion Neurons with Special Focus on Nav1.7, Species Differences, and Regulation by Paclitaxel. Neurosci Bull. 2018 Feb;34(1):4–12.
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    • Yin Y, Wu M, Zubcevic L, Borschel WF, Lander GC, Lee S-Y. Structure of the cold- and menthol-sensing ion channel TRPM8. Science. 2018 Jan 12;359(6372):237–41.
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    • Hirschi M, Herzik MA, Wie J, Suo Y, Borschel WF, Ren D, et al. Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3. Nature. 2017 Oct 19;550(7676):411–4.
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    • Hirschi M, Johnson ZL, Lee S-Y. Visualizing multistep elevator-like transitions of a nucleoside transporter. Nature. 2017 May 4;545(7652):66–70.
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    • Kuk ACY, Mashalidis EH, Lee S-Y. Crystal structure of the MOP flippase MurJ in an inward-facing conformation. Nat Struct Mol Biol. 2017 Feb;24(2):171–6.
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    • Pitt GS, Lee S-Y. Current view on regulation of voltage-gated sodium channels by calcium and auxiliary proteins. Protein Sci. 2016 Sep;25(9):1573–84.
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    • Chung BC, Mashalidis EH, Tanino T, Kim M, Matsuda A, Hong J, et al. Structural insights into inhibition of lipid I production in bacterial cell wall synthesis. Nature. 2016 May 26;533(7604):557–60.
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    • Zubcevic L, Herzik MA, Chung BC, Liu Z, Lander GC, Lee S-Y. Cryo-electron microscopy structure of the TRPV2 ion channel. Nat Struct Mol Biol. 2016 Feb;23(2):180–6.
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    • Johnson ZL, Lee S-Y. Liposome reconstitution and transport assay for recombinant transporters. Methods Enzymol. 2015;556:373–83.
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    • Wang C, Chung BC, Yan H, Wang H-G, Lee S-Y, Pitt GS. Structural analyses of Ca²⁺/CaM interaction with NaV channel C-termini reveal mechanisms of calcium-dependent regulation. Nat Commun. 2014 Sep 18;5:4896.
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    • Johnson ZL, Lee J-H, Lee K, Lee M, Kwon D-Y, Hong J, et al. Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters. Elife. 2014 Jul 31;3:e03604.
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    • Johnson ZL, Lee J-H, Lee K, Lee M, Kwon D-Y, Hong J, et al. Structural basis of nucleoside and nucleoside drug selectivity by concentrative nucleoside transporters. Elife. 2014 Jul 31;3.
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    • Lee J-H, Park C-K, Chen G, Han Q, Xie R-G, Liu T, et al. A monoclonal antibody that targets a NaV1.7 channel voltage sensor for pain and itch relief. Cell. 2014 Jun 5;157(6):1393–404.
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    • Chung BC, Zhao J, Gillespie RA, Kwon D-Y, Guan Z, Hong J, et al. Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis. Science. 2013 Aug 30;341(6149):1012–6.
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    • Chung BC, Zhao J, Gillespie RA, Kwon DY, Guan Z, Hong J, et al. Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis. Science. 2013;341(6149):1012–6.
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    • Wang C, Chung BC, Yan H, Lee S-Y, Pitt GS. Crystal structure of the ternary complex of a NaV C-terminal domain, a fibroblast growth factor homologous factor, and calmodulin. Structure. 2012 Jul 3;20(7):1167–76.
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    • Johnson ZL, Cheong C-G, Lee S-Y. Crystal structure of a concentrative nucleoside transporter from Vibrio cholerae at 2.4 Å. Nature. 2012 Mar 11;483(7390):489–93.
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    • Lee S-Y, Letts JA, MacKinnon R. Functional reconstitution of purified human Hv1 H+ channels. J Mol Biol. 2009 Apr 17;387(5):1055–60.
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    • Lee S-Y, Banerjee A, MacKinnon R. Two separate interfaces between the voltage sensor and pore are required for the function of voltage-dependent K(+) channels. Plos Biol. 2009 Mar 3;7(3):e47.
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    • Lee SY, Banerjee A, Mackinnon R. Two separate interfaces between the Voltage sensor and pore are required for the function of Voltage-dependent K⁺ channels. Plos Biology. 2009 Mar 1;7(3):0676–86.
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    • Lee S-Y, Letts JA, Mackinnon R. Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1. Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7692–5.
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    • Lee S-Y, Lee A, Chen J, MacKinnon R. Structure of the KvAP voltage-dependent K+ channel and its dependence on the lipid membrane. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15441–6.
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    • Kim HS, Damo SM, Lee S-Y, Wemmer D, Klinman JP. Structure and hydride transfer mechanism of a moderate thermophilic dihydrofolate reductase from Bacillus stearothermophilus and comparison to its mesophilic and hyperthermophilic homologues. Biochemistry. 2005 Aug 30;44(34):11428–39.
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    • Roberts A, Lee S-Y, McCullagh E, Silversmith RE, Wemmer DE. YbiV from Escherichia coli K12 is a HAD phosphatase. Proteins. 2005 Mar 1;58(4):790–801.
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    • Lee S-Y, MacKinnon R. A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom. Nature. 2004 Jul 8;430(6996):232–5.
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    • Lee S-Y, De La Torre A, Yan D, Kustu S, Nixon BT, Wemmer DE. Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains. Genes Dev. 2003 Oct 15;17(20):2552–63.
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    • Hastings CA, Lee S-Y, Cho HS, Yan D, Kustu S, Wemmer DE. High-resolution solution structure of the beryllofluoride-activated NtrC receiver domain. Biochemistry. 2003 Aug 5;42(30):9081–90.
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    • Gardino AK, Volkman BF, Cho HS, Lee S-Y, Wemmer DE, Kern D. The NMR solution structure of BeF(3)(-)-activated Spo0F reveals the conformational switch in a phosphorelay system. J Mol Biol. 2003 Aug 1;331(1):245–54.
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    • Rubin SM, Lee S-Y, Ruiz EJ, Pines A, Wemmer DE. Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectroscopy. J Mol Biol. 2002 Sep 13;322(2):425–40.
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    • Lee SY, Cho HS, Pelton JG, Yan D, Berry EA, Wemmer DE. Crystal structure of activated CheY. Comparison with other activated receiver domains. J Biol Chem. 2001 May 11;276(19):16425–31.
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    • Lee SY, Cho HS, Pelton JG, Yan D, Henderson RK, King DS, et al. Crystal structure of an activated response regulator bound to its target. Nat Struct Biol. 2001 Jan;8(1):52–6.
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    • Cho HS, Lee SY, Yan D, Pan X, Parkinson JS, Kustu S, et al. NMR structure of activated CheY. J Mol Biol. 2000 Mar 31;297(3):543–51.
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    • Yan D, Cho HS, Hastings CA, Igo MM, Lee SY, Pelton JG, et al. Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14789–94.
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    • Lee SY, Lee JH, Chang HJ, Cho JM, Jung JW, Lee W. Solution structure of a sweet protein single-chain monellin determined by nuclear magnetic resonance and dynamical simulated annealing calculations. Biochemistry. 1999 Feb 23;38(8):2340–6.
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    • Li HL, Sui HX, Ghanshani S, Lee S, Walian PJ, Wu CL, et al. Two-dimensional crystallization and projection structure of KcsA potassium channel. J Mol Biol. 1998 Sep 18;282(2):211–6.
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  • Book Sections

    • Suo Y, Lee S-Y. Sample preparation of the human TRPA1 ion channel for cryo-EM studies. In 2021. p. 75–87.
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• Teaching & Mentoring
• 

  Recent Courses

  • BIOCHEM 393: Research Independent Study 2022
  • BIOCHEM 593: Research Independent Study 2022
  • BIOCHEM 631: Contemporary Topics in Membrane Biology 2022
  • CMB 631: Contemporary Topics in Membrane Biology 2022
  • NEUROBIO 631: Contemporary Topics in Membrane Biology 2022
  • PHARM 631: Contemporary Topics in Membrane Biology 2022
  • BIOCHEM 393: Research Independent Study 2021
  • BIOCHEM 593: Research Independent Study 2021
  • BIOCHEM 631: Contemporary Topics in Membrane Biology 2021
  • CMB 631: Contemporary Topics in Membrane Biology 2021
  • NEUROBIO 631: Contemporary Topics in Membrane Biology 2021
  • PHARM 631: Contemporary Topics in Membrane Biology 2021
  • BIOCHEM 393: Research Independent Study 2020
  • BIOCHEM 593: Research Independent Study 2020
  • BIOCHEM 631: Contemporary Topics in Membrane Biology 2020
  • CMB 631: Contemporary Topics in Membrane Biology 2020
  • NEUROBIO 631: Contemporary Topics in Membrane Biology 2020
  • PHARM 631: Contemporary Topics in Membrane Biology 2020

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