Muscarinic acetylcholine receptor M3 modulates odorant receptor activity via inhibition of β-arrestin-2 recruitment.
Journal Article
The olfactory system in rodents serves a critical function in social, reproductive and survival behaviours. Processing of chemosensory signals in the brain is dynamically regulated in part by an animal's physiological state. We previously reported that type 3 muscarinic acetylcholine receptors (M3-Rs) physically interact with odorant receptors (ORs) to promote odour-induced responses in a heterologous expression system. However, it is not known how M3-Rs affect the ability of olfactory sensory neurons (OSNs) to respond to odours. Here, we show that an M3-R antagonist attenuates odour-induced responses in OSNs from wild-type, but not M3-R-null, mice. Using a novel molecular assay, we demonstrate that the activation of M3-Rs inhibits the recruitment of β-arrestin-2 to ORs, resulting in a potentiation of odour-induced responses in OSNs. These results suggest a role for acetylcholine in modulating olfactory processing at the initial stages of signal transduction in the olfactory system.
Full Text
Duke Authors
Cited Authors
- Jiang, Y; Li, YR; Tian, H; Ma, M; Matsunami, H
Published Date
- March 24, 2015
Published In
Volume / Issue
- 6 /
Start / End Page
- 6448 -
PubMed ID
- 25800153
Pubmed Central ID
- 25800153
Electronic International Standard Serial Number (EISSN)
- 2041-1723
International Standard Serial Number (ISSN)
- 2041-1723
Digital Object Identifier (DOI)
- 10.1038/ncomms7448
Language
- eng