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Dynamic control and quantification of bacterial population dynamics in droplets.

Publication ,  Journal Article
Huang, S; Srimani, JK; Lee, AJ; Zhang, Y; Lopatkin, AJ; Leong, KW; You, L
Published in: Biomaterials
August 2015

Culturing and measuring bacterial population dynamics are critical to develop insights into gene regulation or bacterial physiology. Traditional methods, based on bulk culture to obtain such quantification, have the limitations of higher cost/volume of reagents, non-amendable to small size of population and more laborious manipulation. To this end, droplet-based microfluidics represents a promising alternative that is cost-effective and high-throughput. However, difficulties in manipulating the droplet environment and monitoring encapsulated bacterial population for long-term experiments limit its utilization. To overcome these limitations, we used an electrode-free injection technology to modulate the chemical environment in droplets. This ability is critical for precise control of bacterial dynamics in droplets. Moreover, we developed a trapping device for long-term monitoring of population dynamics in individual droplets for at least 240 h. We demonstrated the utility of this new microfluidic system by quantifying population dynamics of natural and engineered bacteria. Our approach can further improve the analysis for systems and synthetic biology in terms of manipulability and high temporal resolution.

Duke Scholars

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

August 2015

Volume

61

Start / End Page

239 / 245

Related Subject Headings

  • Micromanipulation
  • Lab-On-A-Chip Devices
  • Flow Injection Analysis
  • Equipment Failure Analysis
  • Equipment Design
  • Cell Separation
  • Bioreactors
  • Biomedical Engineering
  • Batch Cell Culture Techniques
  • Bacteria
 

Citation

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Huang, S., Srimani, J. K., Lee, A. J., Zhang, Y., Lopatkin, A. J., Leong, K. W., & You, L. (2015). Dynamic control and quantification of bacterial population dynamics in droplets. Biomaterials, 61, 239–245. https://doi.org/10.1016/j.biomaterials.2015.05.038
Huang, Shuqiang, Jaydeep K. Srimani, Anna J. Lee, Ying Zhang, Allison J. Lopatkin, Kam W. Leong, and Lingchong You. “Dynamic control and quantification of bacterial population dynamics in droplets.Biomaterials 61 (August 2015): 239–45. https://doi.org/10.1016/j.biomaterials.2015.05.038.
Huang S, Srimani JK, Lee AJ, Zhang Y, Lopatkin AJ, Leong KW, et al. Dynamic control and quantification of bacterial population dynamics in droplets. Biomaterials. 2015 Aug;61:239–45.
Huang, Shuqiang, et al. “Dynamic control and quantification of bacterial population dynamics in droplets.Biomaterials, vol. 61, Aug. 2015, pp. 239–45. Epmc, doi:10.1016/j.biomaterials.2015.05.038.
Huang S, Srimani JK, Lee AJ, Zhang Y, Lopatkin AJ, Leong KW, You L. Dynamic control and quantification of bacterial population dynamics in droplets. Biomaterials. 2015 Aug;61:239–245.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

August 2015

Volume

61

Start / End Page

239 / 245

Related Subject Headings

  • Micromanipulation
  • Lab-On-A-Chip Devices
  • Flow Injection Analysis
  • Equipment Failure Analysis
  • Equipment Design
  • Cell Separation
  • Bioreactors
  • Biomedical Engineering
  • Batch Cell Culture Techniques
  • Bacteria