Quantitative phase imaging with molecular sensitivity using photoacoustic microscopy with a miniature ring transducer.

Journal Article (Journal Article)

We present a dual-modality system for both structural and molecular cell imaging based on coregistered quantitative phase imaging (QPI) and photoacoustic microscopy (PAM). The QPI system was based on off-axis holography, whereas the PAM system comprised a sinusoidally modulated optical source for excitation and a narrow-band low profile and low-cost ring ultrasonic transducer for detection. This approach facilitated a simple confocal alignment of the excitation beams of both modalities and the ultrasonic detector. This system was demonstrated by imaging endogenous molecules in red blood cells (RBCs) as well as by imaging exogenous molecular labels on cancer cells using gold nanoparticles (GNPs) functionalized to target epidermal growth factor receptor. QPI provided high resolution imaging of the cellular structures while PAM provided molecular contrast. This dual-modality microscopy method can potentially be implemented as a compact and low cost cellular diagnostic assay.

Full Text

Duke Authors

Cited Authors

  • Sheinfeld, A; Eldridge, WJ; Wax, A

Published Date

  • August 2015

Published In

Volume / Issue

  • 20 / 8

Start / End Page

  • 86002 -

PubMed ID

  • 26263416

Electronic International Standard Serial Number (EISSN)

  • 1560-2281

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.jbo.20.8.086002


  • eng