Constructing atomic-resolution RNA structural ensembles using MD and motionally decoupled NMR RDCs.
Publication
, Journal Article
Stelzer, AC; Frank, AT; Bailor, MH; Andricioaei, I; Al-Hashimi, HM
Published in: Methods
October 2009
A broad structural landscape often needs to be characterized in order to fully understand how regulatory RNAs perform their biological functions at the atomic level. We present a protocol for visualizing thermally accessible RNA conformations at atomic-resolution and with timescales extending up to milliseconds. The protocol combines molecular dynamics (MD) simulations with experimental residual dipolar couplings (RDCs) measured in partially aligned (13)C/(15)N isotopically enriched elongated RNA samples. The structural ensembles generated in this manner provide insights into RNA dynamics and its role in functionally important transitions.
Duke Scholars
Published In
Methods
DOI
EISSN
1095-9130
Publication Date
October 2009
Volume
49
Issue
2
Start / End Page
167 / 173
Location
United States
Related Subject Headings
- Transcription, Genetic
- Time Factors
- RNA, Viral
- RNA
- Nucleic Acid Conformation
- Nitrogen Isotopes
- Motion
- Molecular Sequence Data
- Molecular Conformation
- Models, Statistical
Citation
APA
Chicago
ICMJE
MLA
NLM
Stelzer, A. C., Frank, A. T., Bailor, M. H., Andricioaei, I., & Al-Hashimi, H. M. (2009). Constructing atomic-resolution RNA structural ensembles using MD and motionally decoupled NMR RDCs. Methods, 49(2), 167–173. https://doi.org/10.1016/j.ymeth.2009.08.006
Stelzer, Andrew C., Aaron T. Frank, Maximillian H. Bailor, Ioan Andricioaei, and Hashim M. Al-Hashimi. “Constructing atomic-resolution RNA structural ensembles using MD and motionally decoupled NMR RDCs.” Methods 49, no. 2 (October 2009): 167–73. https://doi.org/10.1016/j.ymeth.2009.08.006.
Stelzer AC, Frank AT, Bailor MH, Andricioaei I, Al-Hashimi HM. Constructing atomic-resolution RNA structural ensembles using MD and motionally decoupled NMR RDCs. Methods. 2009 Oct;49(2):167–73.
Stelzer, Andrew C., et al. “Constructing atomic-resolution RNA structural ensembles using MD and motionally decoupled NMR RDCs.” Methods, vol. 49, no. 2, Oct. 2009, pp. 167–73. Pubmed, doi:10.1016/j.ymeth.2009.08.006.
Stelzer AC, Frank AT, Bailor MH, Andricioaei I, Al-Hashimi HM. Constructing atomic-resolution RNA structural ensembles using MD and motionally decoupled NMR RDCs. Methods. 2009 Oct;49(2):167–173.
Published In
Methods
DOI
EISSN
1095-9130
Publication Date
October 2009
Volume
49
Issue
2
Start / End Page
167 / 173
Location
United States
Related Subject Headings
- Transcription, Genetic
- Time Factors
- RNA, Viral
- RNA
- Nucleic Acid Conformation
- Nitrogen Isotopes
- Motion
- Molecular Sequence Data
- Molecular Conformation
- Models, Statistical