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Probing motions between equivalent RNA domains using magnetic field induced residual dipolar couplings: accounting for correlations between motions and alignment.

Publication ,  Journal Article
Zhang, Q; Throolin, R; Pitt, SW; Serganov, A; Al-Hashimi, HM
Published in: J Am Chem Soc
September 3, 2003

Approaches developed thus for extracting structural and dynamical information from RDCs have rested on the assumption that motions do not affect molecular alignment. However, it is well established that molecular alignment in ordered media is dependent on conformation, and slowly interconverting conformational substates may exhibit different alignment properties. Neglecting these correlation effects can lead to aberrations in the structural and dynamical analysis of RDCs and diminish the utility of RDCs in probing motions between domains having similar alignment propensities. Here, we introduce a new approach based on measurement of magnetic field induced residual dipolar couplings in nucleic acids which can explicitly take into account such correlations and demonstrate measurements of motions between two "magnetically equivalent" domains in the transactivation response element (TAR) RNA.

Duke Scholars

Published In

J Am Chem Soc

DOI

ISSN

0002-7863

Publication Date

September 3, 2003

Volume

125

Issue

35

Start / End Page

10530 / 10531

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Response Elements
  • RNA
  • Nucleic Acid Conformation
  • Nuclear Magnetic Resonance, Biomolecular
  • Magnetics
  • General Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 03 Chemical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, Q., Throolin, R., Pitt, S. W., Serganov, A., & Al-Hashimi, H. M. (2003). Probing motions between equivalent RNA domains using magnetic field induced residual dipolar couplings: accounting for correlations between motions and alignment. J Am Chem Soc, 125(35), 10530–10531. https://doi.org/10.1021/ja0363056
Zhang, Qi, Rachel Throolin, Stephen W. Pitt, Alexander Serganov, and Hashim M. Al-Hashimi. “Probing motions between equivalent RNA domains using magnetic field induced residual dipolar couplings: accounting for correlations between motions and alignment.J Am Chem Soc 125, no. 35 (September 3, 2003): 10530–31. https://doi.org/10.1021/ja0363056.
Zhang, Qi, et al. “Probing motions between equivalent RNA domains using magnetic field induced residual dipolar couplings: accounting for correlations between motions and alignment.J Am Chem Soc, vol. 125, no. 35, Sept. 2003, pp. 10530–31. Pubmed, doi:10.1021/ja0363056.
Journal cover image

Published In

J Am Chem Soc

DOI

ISSN

0002-7863

Publication Date

September 3, 2003

Volume

125

Issue

35

Start / End Page

10530 / 10531

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Response Elements
  • RNA
  • Nucleic Acid Conformation
  • Nuclear Magnetic Resonance, Biomolecular
  • Magnetics
  • General Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 03 Chemical Sciences