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Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus.

Publication ,  Journal Article
Liu, J; Ochieng, C; Wiersma, S; Ströher, U; Towner, JS; Whitmer, S; Nichol, ST; Moore, CC; Kersh, GJ; Kato, C; Sexton, C; Petersen, J ...
Published in: J Clin Microbiol
January 2016

Acute febrile illness (AFI) is associated with substantial morbidity and mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, and many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results and can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan and Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, and yellow fever virus), 8 bacteria (Bartonella spp., Brucella spp., Coxiella burnetii, Leptospira spp., Rickettsia spp., Salmonella enterica and Salmonella enterica serovar Typhi, and Yersinia pestis), and 3 protozoa (Leishmania spp., Plasmodium spp., and Trypanosoma brucei). Two extrinsic controls (phocine herpesvirus 1 and bacteriophage MS2) were included to ensure extraction and amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, and specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/315; 95% confidence interval [CI], 84% to 92%) sensitivity and a 99% (5,261/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.

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Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

January 2016

Volume

54

Issue

1

Start / End Page

49 / 58

Location

United States

Related Subject Headings

  • Time Factors
  • Sensitivity and Specificity
  • Reference Standards
  • Real-Time Polymerase Chain Reaction
  • Molecular Diagnostic Techniques
  • Microbiology
  • Humans
  • Fever of Unknown Origin
  • Epidemiological Monitoring
  • Disease Outbreaks
 

Citation

APA
Chicago
ICMJE
MLA
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Liu, J., Ochieng, C., Wiersma, S., Ströher, U., Towner, J. S., Whitmer, S., … Fields, B. (2016). Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus. J Clin Microbiol, 54(1), 49–58. https://doi.org/10.1128/JCM.02257-15
Liu, Jie, Caroline Ochieng, Steve Wiersma, Ute Ströher, Jonathan S. Towner, Shannon Whitmer, Stuart T. Nichol, et al. “Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus.J Clin Microbiol 54, no. 1 (January 2016): 49–58. https://doi.org/10.1128/JCM.02257-15.
Liu J, Ochieng C, Wiersma S, Ströher U, Towner JS, Whitmer S, et al. Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus. J Clin Microbiol. 2016 Jan;54(1):49–58.
Liu, Jie, et al. “Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus.J Clin Microbiol, vol. 54, no. 1, Jan. 2016, pp. 49–58. Pubmed, doi:10.1128/JCM.02257-15.
Liu J, Ochieng C, Wiersma S, Ströher U, Towner JS, Whitmer S, Nichol ST, Moore CC, Kersh GJ, Kato C, Sexton C, Petersen J, Massung R, Hercik C, Crump JA, Kibiki G, Maro A, Mujaga B, Gratz J, Jacob ST, Banura P, Scheld WM, Juma B, Onyango CO, Montgomery JM, Houpt E, Fields B. Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus. J Clin Microbiol. 2016 Jan;54(1):49–58.

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

January 2016

Volume

54

Issue

1

Start / End Page

49 / 58

Location

United States

Related Subject Headings

  • Time Factors
  • Sensitivity and Specificity
  • Reference Standards
  • Real-Time Polymerase Chain Reaction
  • Molecular Diagnostic Techniques
  • Microbiology
  • Humans
  • Fever of Unknown Origin
  • Epidemiological Monitoring
  • Disease Outbreaks