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Regulation of interleukin-8 expression by cellular pH in human pancreatic adenocarcinoma cells.

Publication ,  Journal Article
Shi, Q; Le, X; Wang, B; Xiong, Q; Abbruzzese, JL; Xie, K
Published in: J Interferon Cytokine Res
November 2000

The role of cellular pH in the expression and regulation of interleukin-8 (IL-8) in human tumor cell lines was determined. Transient exposure to pH ranging from 7.4 to 6.7 induced pH-dependent expression of IL-8 at both the mRNA and protein levels in three different human tumor cell lines, including COLO357 pancreatic adenocarcinoma cells, SW620 colon adenocarcinoma cells, and PC3 prostate adenocarcinoma cells. Investigation of the mechanisms of IL-8 induction in response to acidosis was carried out using the COLO357 human pancreatic cancer cell line. The increased steady-state level of mRNA correlated with an increased transcription rate and stability of IL-8 transcripts. Further experiments indicated that mild acidosis activated the transcription factors NF-kappaB and AP-1 and that the cooperation of these two factors appeared to be essential to the transactivation of the IL-8 gene. Our data demonstrated that low tumor pH contributes to the enhanced expression of IL-8 and plays an important role in tumor progression.

Duke Scholars

Published In

J Interferon Cytokine Res

DOI

ISSN

1079-9907

Publication Date

November 2000

Volume

20

Issue

11

Start / End Page

1023 / 1028

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcriptional Activation
  • Transcription, Genetic
  • Transcription Factor AP-1
  • RNA, Messenger
  • RNA Stability
  • Promoter Regions, Genetic
  • Pancreatic Neoplasms
  • NF-kappa B
  • Interleukin-8
 

Citation

APA
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ICMJE
MLA
NLM
Shi, Q., Le, X., Wang, B., Xiong, Q., Abbruzzese, J. L., & Xie, K. (2000). Regulation of interleukin-8 expression by cellular pH in human pancreatic adenocarcinoma cells. J Interferon Cytokine Res, 20(11), 1023–1028. https://doi.org/10.1089/10799900050198471
Shi, Q., X. Le, B. Wang, Q. Xiong, J. L. Abbruzzese, and K. Xie. “Regulation of interleukin-8 expression by cellular pH in human pancreatic adenocarcinoma cells.J Interferon Cytokine Res 20, no. 11 (November 2000): 1023–28. https://doi.org/10.1089/10799900050198471.
Shi Q, Le X, Wang B, Xiong Q, Abbruzzese JL, Xie K. Regulation of interleukin-8 expression by cellular pH in human pancreatic adenocarcinoma cells. J Interferon Cytokine Res. 2000 Nov;20(11):1023–8.
Shi, Q., et al. “Regulation of interleukin-8 expression by cellular pH in human pancreatic adenocarcinoma cells.J Interferon Cytokine Res, vol. 20, no. 11, Nov. 2000, pp. 1023–28. Pubmed, doi:10.1089/10799900050198471.
Shi Q, Le X, Wang B, Xiong Q, Abbruzzese JL, Xie K. Regulation of interleukin-8 expression by cellular pH in human pancreatic adenocarcinoma cells. J Interferon Cytokine Res. 2000 Nov;20(11):1023–1028.
Journal cover image

Published In

J Interferon Cytokine Res

DOI

ISSN

1079-9907

Publication Date

November 2000

Volume

20

Issue

11

Start / End Page

1023 / 1028

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcriptional Activation
  • Transcription, Genetic
  • Transcription Factor AP-1
  • RNA, Messenger
  • RNA Stability
  • Promoter Regions, Genetic
  • Pancreatic Neoplasms
  • NF-kappa B
  • Interleukin-8