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Establishment of three-dimensional cultures of human pancreatic duct epithelial cells.

Publication ,  Journal Article
Gutierrez-Barrera, AM; Menter, DG; Abbruzzese, JL; Reddy, SAG
Published in: Biochem Biophys Res Commun
July 6, 2007

Three-dimensional (3D) cultures of epithelial cells offer singular advantages for studies of morphogenesis or the role of cancer genes in oncogenesis. In this study, as part of establishing a 3D culture system of pancreatic duct epithelial cells, we compared human pancreatic duct epithelial cells (HPDE-E6E7) with pancreatic cancer cell lines. Our results show, that in contrast to cancer cells, HPDE-E6E7 organized into spheroids with what appeared to be apical and basal membranes and a luminal space. Immunostaining experiments indicated that protein kinase Akt was phosphorylated (Ser473) and CTMP, a negative Akt regulator, was expressed in both HPDE-E6E7 and cancer cells. However, a nuclear pool of CTMP was detectable in HPDE-E6E7 cells that showed a dynamic concentrated expression pattern, a feature that further distinguished HPDE-E637 cells from cancer cells. Collectively, these data suggest that 3D cultures of HPDE-E6E7 cells are useful for investigating signaling and morphological abnormalities in pancreatic cancer cells.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

July 6, 2007

Volume

358

Issue

3

Start / End Page

698 / 703

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Signal Transduction
  • Proto-Oncogene Proteins c-akt
  • Proteoglycans
  • Phosphorylation
  • Pancreatic Neoplasms
  • Pancreatic Ducts
  • Microscopy, Fluorescence
  • Laminin
  • Humans
 

Citation

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Chicago
ICMJE
MLA
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Gutierrez-Barrera, A. M., Menter, D. G., Abbruzzese, J. L., & Reddy, S. A. G. (2007). Establishment of three-dimensional cultures of human pancreatic duct epithelial cells. Biochem Biophys Res Commun, 358(3), 698–703. https://doi.org/10.1016/j.bbrc.2007.04.166
Gutierrez-Barrera, Angelica M., David G. Menter, James L. Abbruzzese, and Shrikanth A. G. Reddy. “Establishment of three-dimensional cultures of human pancreatic duct epithelial cells.Biochem Biophys Res Commun 358, no. 3 (July 6, 2007): 698–703. https://doi.org/10.1016/j.bbrc.2007.04.166.
Gutierrez-Barrera AM, Menter DG, Abbruzzese JL, Reddy SAG. Establishment of three-dimensional cultures of human pancreatic duct epithelial cells. Biochem Biophys Res Commun. 2007 Jul 6;358(3):698–703.
Gutierrez-Barrera, Angelica M., et al. “Establishment of three-dimensional cultures of human pancreatic duct epithelial cells.Biochem Biophys Res Commun, vol. 358, no. 3, July 2007, pp. 698–703. Pubmed, doi:10.1016/j.bbrc.2007.04.166.
Gutierrez-Barrera AM, Menter DG, Abbruzzese JL, Reddy SAG. Establishment of three-dimensional cultures of human pancreatic duct epithelial cells. Biochem Biophys Res Commun. 2007 Jul 6;358(3):698–703.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

July 6, 2007

Volume

358

Issue

3

Start / End Page

698 / 703

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Signal Transduction
  • Proto-Oncogene Proteins c-akt
  • Proteoglycans
  • Phosphorylation
  • Pancreatic Neoplasms
  • Pancreatic Ducts
  • Microscopy, Fluorescence
  • Laminin
  • Humans