Establishment of three-dimensional cultures of human pancreatic duct epithelial cells.
Journal Article (Journal Article)
Three-dimensional (3D) cultures of epithelial cells offer singular advantages for studies of morphogenesis or the role of cancer genes in oncogenesis. In this study, as part of establishing a 3D culture system of pancreatic duct epithelial cells, we compared human pancreatic duct epithelial cells (HPDE-E6E7) with pancreatic cancer cell lines. Our results show, that in contrast to cancer cells, HPDE-E6E7 organized into spheroids with what appeared to be apical and basal membranes and a luminal space. Immunostaining experiments indicated that protein kinase Akt was phosphorylated (Ser473) and CTMP, a negative Akt regulator, was expressed in both HPDE-E6E7 and cancer cells. However, a nuclear pool of CTMP was detectable in HPDE-E6E7 cells that showed a dynamic concentrated expression pattern, a feature that further distinguished HPDE-E637 cells from cancer cells. Collectively, these data suggest that 3D cultures of HPDE-E6E7 cells are useful for investigating signaling and morphological abnormalities in pancreatic cancer cells.
Full Text
Duke Authors
Cited Authors
- Gutierrez-Barrera, AM; Menter, DG; Abbruzzese, JL; Reddy, SAG
Published Date
- July 6, 2007
Published In
Volume / Issue
- 358 / 3
Start / End Page
- 698 - 703
PubMed ID
- 17512909
Pubmed Central ID
- PMC2562614
International Standard Serial Number (ISSN)
- 0006-291X
Digital Object Identifier (DOI)
- 10.1016/j.bbrc.2007.04.166
Language
- eng
Conference Location
- United States