Integration Site Choice of a Feline Immunodeficiency Virus Vector

Journal Article

ABSTRACT We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a “bendable” structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.

Full Text

Duke Authors

Cited Authors

  • Kang, Y; Moressi, CJ; Scheetz, TE; Xie, L; Tran, DT; Casavant, TL; Ak, P; Benham, CJ; Davidson, BL; McCray, PB

Published Date

  • September 2006

Published In

Volume / Issue

  • 80 / 17

Start / End Page

  • 8820 - 8823

Published By

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.00719-06

Language

  • en