Expression of amphiphysin I, an autoantigen of paraneoplastic neurological syndromes, in breast cancer.

Published

Journal Article

Amphiphysin I is a 128 kD protein highly concentrated in nerve terminals, where it has a putative role in endocytosis. It is a dominant autoantigen in patients with stiff-man syndrome associated with breast cancer, as well as in other paraneoplastic autoimmune neurological disorders. To elucidate the connection between amphiphysin I autoimmunity and cancer, we investigated its expression in breast cancer tissue. We report that amphiphysin I was expressed as two isoforms of 128 and 108 kD in the breast cancer of a patient with anti-amphiphysin I antibodies and paraneoplastic sensory neuronopathy. Amphiphysin I was also detectable at variable levels in several other human breast cancer tissues and cell lines and at low levels in normal mammary tissue and a variety of other non-neuronal tissues. The predominant amphiphysin I isoform expressed outside the brain in humans is the 108 kD isoform which represents an alternatively spliced variant of neuronal amphiphysin I missing a 42 amino acid insert. Our study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells.

Full Text

Duke Authors

Cited Authors

  • Floyd, S; Butler, MH; Cremona, O; David, C; Freyberg, Z; Zhang, X; Solimena, M; Tokunaga, A; Ishizu, H; Tsutsui, K; De Camilli, P

Published Date

  • January 1998

Published In

Volume / Issue

  • 4 / 1

Start / End Page

  • 29 - 39

PubMed ID

  • 9513187

Pubmed Central ID

  • 9513187

Electronic International Standard Serial Number (EISSN)

  • 1528-3658

International Standard Serial Number (ISSN)

  • 1076-1551

Digital Object Identifier (DOI)

  • 10.1007/bf03401727

Language

  • eng