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Expression of amphiphysin I, an autoantigen of paraneoplastic neurological syndromes, in breast cancer.

Publication ,  Journal Article
Floyd, S; Butler, MH; Cremona, O; David, C; Freyberg, Z; Zhang, X; Solimena, M; Tokunaga, A; Ishizu, H; Tsutsui, K; De Camilli, P
Published in: Mol Med
January 1998

Amphiphysin I is a 128 kD protein highly concentrated in nerve terminals, where it has a putative role in endocytosis. It is a dominant autoantigen in patients with stiff-man syndrome associated with breast cancer, as well as in other paraneoplastic autoimmune neurological disorders. To elucidate the connection between amphiphysin I autoimmunity and cancer, we investigated its expression in breast cancer tissue. We report that amphiphysin I was expressed as two isoforms of 128 and 108 kD in the breast cancer of a patient with anti-amphiphysin I antibodies and paraneoplastic sensory neuronopathy. Amphiphysin I was also detectable at variable levels in several other human breast cancer tissues and cell lines and at low levels in normal mammary tissue and a variety of other non-neuronal tissues. The predominant amphiphysin I isoform expressed outside the brain in humans is the 108 kD isoform which represents an alternatively spliced variant of neuronal amphiphysin I missing a 42 amino acid insert. Our study suggests a link between amphiphysin I expression in cancer and amphiphysin I autoimmunity. The enhanced expression of amphiphysin I in some forms of cancer supports the hypothesis that amphiphysin family members may play a role in the biology of cancer cells.

Duke Scholars

Published In

Mol Med

ISSN

1076-1551

Publication Date

January 1998

Volume

4

Issue

1

Start / End Page

29 / 39

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Stiff-Person Syndrome
  • Rats
  • Protein Biosynthesis
  • Polymerase Chain Reaction
  • Nervous System Diseases
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Isomerism
  • Immunology
 

Citation

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MLA
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Floyd, S., Butler, M. H., Cremona, O., David, C., Freyberg, Z., Zhang, X., … De Camilli, P. (1998). Expression of amphiphysin I, an autoantigen of paraneoplastic neurological syndromes, in breast cancer. Mol Med, 4(1), 29–39.
Floyd, S., M. H. Butler, O. Cremona, C. David, Z. Freyberg, X. Zhang, M. Solimena, et al. “Expression of amphiphysin I, an autoantigen of paraneoplastic neurological syndromes, in breast cancer.Mol Med 4, no. 1 (January 1998): 29–39.
Floyd S, Butler MH, Cremona O, David C, Freyberg Z, Zhang X, et al. Expression of amphiphysin I, an autoantigen of paraneoplastic neurological syndromes, in breast cancer. Mol Med. 1998 Jan;4(1):29–39.
Floyd, S., et al. “Expression of amphiphysin I, an autoantigen of paraneoplastic neurological syndromes, in breast cancer.Mol Med, vol. 4, no. 1, Jan. 1998, pp. 29–39.
Floyd S, Butler MH, Cremona O, David C, Freyberg Z, Zhang X, Solimena M, Tokunaga A, Ishizu H, Tsutsui K, De Camilli P. Expression of amphiphysin I, an autoantigen of paraneoplastic neurological syndromes, in breast cancer. Mol Med. 1998 Jan;4(1):29–39.

Published In

Mol Med

ISSN

1076-1551

Publication Date

January 1998

Volume

4

Issue

1

Start / End Page

29 / 39

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Stiff-Person Syndrome
  • Rats
  • Protein Biosynthesis
  • Polymerase Chain Reaction
  • Nervous System Diseases
  • Nerve Tissue Proteins
  • Molecular Sequence Data
  • Isomerism
  • Immunology