SV40 enhancer-binding factors are required at the establishment but not the maintenance step of enhancer-dependent transcriptional activation.

Journal Article

We have used temperature-sensitive COS cells to design delayed competition experiments in which competition for simian virus 40 (SV40) enhancer factors occurs after enhancer-dependent transcription has been established. The results demonstrate that competition for SV40 enhancer-binding factors has no effect on enhancer-dependent transcription after transcription has been established at the SV40 early promoter. These data show that the enhancer and factors that bind to it are involved in the establishment of stable transcription complexes, although they do not show whether enhancer factors are an integral part of the transcription complexes. Furthermore, the results of delayed competition experiments with a replicating test plasmid are consistent with the possibility that enhancer-dependent stable transcription complexes could be maintained after DNA replication.

Full Text

Duke Authors

Cited Authors

  • Wang, XF; Calame, K

Published Date

  • October 1, 1986

Published In

Volume / Issue

  • 47 / 2

Start / End Page

  • 241 - 247

PubMed ID

  • 3021335

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(86)90446-0

Language

  • eng