Multiphasic effect of morphine on the release of substance P from rat trigeminal nucleus slices.

Published

Journal Article

It is generally accepted that morphine acts presynaptically to inhibit substance P (SP) release from afferent terminals in the trigeminal nucleus. Recent studies, however, provide evidence that opioids produce both inhibitory and excitatory effects on SP release which are concentration- and receptor subtype-dependent. In the present study, we have examined a wide range of morphine concentrations on K(+)-evoked SP release from rat trigeminal nucleus caudalis slices. Immunoreactive SP was measured in perfusates. Morphine produced multiphasic effects on K(+)-evoked SP release without affecting basal release. A very low nanomolar concentration (1 nM) suppressed release, higher nanomolar concentrations (100-300 nM) facilitated release, a low micromolar concentration (3 microM) suppressed release, and a higher micromolar concentration (30 microM) facilitated release. These effects were abolished by opioid receptor blockade with naloxone (30 nM). Thus, morphine produces a complex bi-directional modulation of SP release from TNC which is concentration- and possibly receptor subtype-dependent.

Full Text

Duke Authors

Cited Authors

  • Suarez-Roca, H; Abdullah, L; Zuniga, J; Madison, S; Maixner, W

Published Date

  • May 8, 1992

Published In

Volume / Issue

  • 579 / 2

Start / End Page

  • 187 - 194

PubMed ID

  • 1378346

Pubmed Central ID

  • 1378346

International Standard Serial Number (ISSN)

  • 0006-8993

Digital Object Identifier (DOI)

  • 10.1016/0006-8993(92)90050-j

Language

  • eng

Conference Location

  • Netherlands