An STS-based map of the human genome.

Journal Article (Journal Article)

A physical map has been constructed of the human genome containing 15,086 sequence-tagged sites (STSs), with an average spacing of 199 kilobases. The project involved assembly of a radiation hybrid map of the human genome containing 6193 loci and incorporated a genetic linkage map of the human genome containing 5264 loci. This information was combined with the results of STS-content screening of 10,850 loci against a yeast artificial chromosome library to produce an integrated map, anchored by the radiation hybrid and genetic maps. The map provides radiation hybrid coverage of 99 percent and physical coverage of 94 percent of the human genome. The map also represents an early step in an international project to generate a transcript map of the human genome, with more than 3235 expressed sequences localized. The STSs in the map provide a scaffold for initiating large-scale sequencing of the human genome.

Full Text

Duke Authors

Cited Authors

  • Hudson, TJ; Stein, LD; Gerety, SS; Ma, J; Castle, AB; Silva, J; Slonim, DK; Baptista, R; Kruglyak, L; Xu, SH; Hu, X; Colbert, AM; Rosenberg, C; Reeve-Daly, MP; Rozen, S; Hui, L; Wu, X; Vestergaard, C; Wilson, KM; Bae, JS; Maitra, S; Ganiatsas, S; Evans, CA; DeAngelis, MM; Ingalls, KA; Nahf, RW; Horton, LT; Anderson, MO; Collymore, AJ; Ye, W; Kouyoumjian, V; Zemsteva, IS; Tam, J; Devine, R; Courtney, DF; Renaud, MT; Nguyen, H; O'Connor, TJ; Fizames, C; Fauré, S; Gyapay, G; Dib, C; Morissette, J; Orlin, JB; Birren, BW; Goodman, N; Weissenbach, J; Hawkins, TL; Foote, S; Page, DC; Lander, ES

Published Date

  • December 22, 1995

Published In

Volume / Issue

  • 270 / 5244

Start / End Page

  • 1945 - 1954

PubMed ID

  • 8533086

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.270.5244.1945


  • eng

Conference Location

  • United States