Microarray analysis of peroxisome proliferator-activated receptor-gamma induced changes in gene expression in macrophages.

Journal Article (Journal Article)

We used a combination of expression microarray and Northern blot analyses to identify target genes for peroxisome proliferator-activated receptor (PPAR) gamma in RAW264.7 macrophages. PPARgamma natural ligand 15-deoxy-Delta(12,14) prostaglandin and synthetic ligands ciglitazone and rosiglitazone increased the expression of scavenger receptor CD36 and ATP-binding cassette transporter A1, as well as adipophilin (a lipid droplet coating protein involved in intracellular lipid storage and transport), calpain (a protease implicated in ABCA1 protein degradation), and ADAM8 (a disintegrin and metalloprotease protein involved in cell adhesion). These findings are relevant to understanding the effect of PPARgamma activation on gene expression and cognate pathways in macrophages.

Full Text

Duke Authors

Cited Authors

  • Hodgkinson, CP; Ye, S

Published Date

  • August 29, 2003

Published In

Volume / Issue

  • 308 / 3

Start / End Page

  • 505 - 510

PubMed ID

  • 12914779

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/s0006-291x(03)01416-5


  • eng

Conference Location

  • United States