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Identification of the yeast cytidine deaminase CDD1 as an orphan C-->U RNA editase.

Publication ,  Journal Article
Dance, GS; Beemiller, P; Yang, Y; Mater, DV; Mian, IS; Smith, HC
Published in: Nucleic Acids Res
April 15, 2001

Yeast co-expressing rat APOBEC-1 and a fragment of human apolipoprotein B (apoB) mRNA assembled functional editosomes and deaminated C6666 to U in a mooring sequence-dependent fashion. The occurrence of APOBEC-1-complementing proteins suggested a naturally occurring mRNA editing mechanism in yeast. Previously, a hidden Markov model identified seven yeast genes encoding proteins possessing putative zinc-dependent deaminase motifs. Here, only CDD1, a cytidine deaminase, is shown to have the capacity to carry out C-->U editing on a reporter mRNA. This is only the second report of a cytidine deaminase that can use mRNA as a substrate. CDD1-dependent editing was growth phase regulated and demonstrated mooring sequence-dependent editing activity. Candidate yeast mRNA substrates were identified based on their homology with the mooring sequence-containing tripartite motif at the editing site of apoB mRNA and their ability to be edited by ectopically expressed APOBEC-1. Naturally occurring yeast mRNAs edited to a significant extent by CDD1 were, however, not detected. We propose that CDD1 be designated an orphan C-->U editase until its native RNA substrate, if any, can be identified and that it be added to the CDAR (cytidine deaminase acting on RNA) family of editing enzymes.

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Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

April 15, 2001

Volume

29

Issue

8

Start / End Page

1772 / 1780

Location

England

Related Subject Headings

  • Yeasts
  • Sequence Alignment
  • Recombinant Fusion Proteins
  • Rats
  • RNA, Messenger
  • RNA, Fungal
  • RNA Editing
  • Protein Structure, Tertiary
  • Open Reading Frames
  • Molecular Sequence Data
 

Citation

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Dance, G. S., Beemiller, P., Yang, Y., Mater, D. V., Mian, I. S., & Smith, H. C. (2001). Identification of the yeast cytidine deaminase CDD1 as an orphan C-->U RNA editase. Nucleic Acids Res, 29(8), 1772–1780. https://doi.org/10.1093/nar/29.8.1772
Dance, G. S., P. Beemiller, Y. Yang, D. V. Mater, I. S. Mian, and H. C. Smith. “Identification of the yeast cytidine deaminase CDD1 as an orphan C-->U RNA editase.Nucleic Acids Res 29, no. 8 (April 15, 2001): 1772–80. https://doi.org/10.1093/nar/29.8.1772.
Dance GS, Beemiller P, Yang Y, Mater DV, Mian IS, Smith HC. Identification of the yeast cytidine deaminase CDD1 as an orphan C-->U RNA editase. Nucleic Acids Res. 2001 Apr 15;29(8):1772–80.
Dance, G. S., et al. “Identification of the yeast cytidine deaminase CDD1 as an orphan C-->U RNA editase.Nucleic Acids Res, vol. 29, no. 8, Apr. 2001, pp. 1772–80. Pubmed, doi:10.1093/nar/29.8.1772.
Dance GS, Beemiller P, Yang Y, Mater DV, Mian IS, Smith HC. Identification of the yeast cytidine deaminase CDD1 as an orphan C-->U RNA editase. Nucleic Acids Res. 2001 Apr 15;29(8):1772–1780.
Journal cover image

Published In

Nucleic Acids Res

DOI

EISSN

1362-4962

Publication Date

April 15, 2001

Volume

29

Issue

8

Start / End Page

1772 / 1780

Location

England

Related Subject Headings

  • Yeasts
  • Sequence Alignment
  • Recombinant Fusion Proteins
  • Rats
  • RNA, Messenger
  • RNA, Fungal
  • RNA Editing
  • Protein Structure, Tertiary
  • Open Reading Frames
  • Molecular Sequence Data