Low K+ promotes NF-kappaB/DNA binding in neuronal apoptosis induced by K+ loss.

Journal Article

Low intracellular K+ concentration ([K+]i) promotes apoptosis and blocking K+ loss prevents apoptosis, but the mechanism of action of low [K+]i remains unclear. Here, we show that low [K+]i increases NF-kappaB transcriptional activity by enhancing its binding to the promoter of target genes without affecting its activation and nuclear translocation in cortical neurons deprived of serum. Low K+ concentration promotes NF-kappaB/DNA binding through direct effects on the interaction of NF-kappaB dimers with DNA. Up-regulation of proapoptotic protein Bcl-XS and neuronal apoptosis induced by serum deprivation are blocked by inhibition and/or down-regulation of NF-kappaB and by prevention of K+ loss. Thus, a direct action of K+ on NF-kappaB/DNA binding regulates gene transcription related to neuronal apoptosis.

Full Text

Duke Authors

Cited Authors

  • Tao, Y; Yan, D; Yang, Q; Zeng, R; Wang, Y

Published Date

  • February 2006

Published In

Volume / Issue

  • 26 / 3

Start / End Page

  • 1038 - 1050

PubMed ID

  • 16428456

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.26.3.1038-1050.2006

Language

  • eng