Low K+ promotes NF-kappaB/DNA binding in neuronal apoptosis induced by K+ loss.
Journal Article (Journal Article)
Low intracellular K+ concentration ([K+]i) promotes apoptosis and blocking K+ loss prevents apoptosis, but the mechanism of action of low [K+]i remains unclear. Here, we show that low [K+]i increases NF-kappaB transcriptional activity by enhancing its binding to the promoter of target genes without affecting its activation and nuclear translocation in cortical neurons deprived of serum. Low K+ concentration promotes NF-kappaB/DNA binding through direct effects on the interaction of NF-kappaB dimers with DNA. Up-regulation of proapoptotic protein Bcl-XS and neuronal apoptosis induced by serum deprivation are blocked by inhibition and/or down-regulation of NF-kappaB and by prevention of K+ loss. Thus, a direct action of K+ on NF-kappaB/DNA binding regulates gene transcription related to neuronal apoptosis.
Full Text
Duke Authors
Cited Authors
- Tao, Y; Yan, D; Yang, Q; Zeng, R; Wang, Y
Published Date
- February 2006
Published In
Volume / Issue
- 26 / 3
Start / End Page
- 1038 - 1050
PubMed ID
- 16428456
Pubmed Central ID
- PMC1347023
International Standard Serial Number (ISSN)
- 0270-7306
Digital Object Identifier (DOI)
- 10.1128/MCB.26.3.1038-1050.2006
Language
- eng
Conference Location
- United States