Hydrodynamic chromatography for the size classification of micron and sub-micron sized packing materials.


Journal Article

Hydrodynamic chromatography (HDC) was used as a size classification and purification method for porous bridged ethyl hybrid (BEH) packing materials (particles) in the micron to sub-micron range. Using packed column HDC, a batch of particles with size 0.76+/-0.26microm was fractionated to yield classified material of 1.05+/-0.16microm, reducing the relative standard deviation from 33% to 15%. Subsequent chromatographic evaluation of this packing material showed significant improvement in column performance and decrease in flow resistance over the unclassified material. Comparing a column packed with the classified versus non-classified material, the effective flow resistance of the two columns was decreased by 58% and the minimum HETP for the packing material was improved from 4 to 2.5microm.

Full Text

Duke Authors

Cited Authors

  • Thompson, JW; Lieberman, RA; Jorgenson, JW

Published Date

  • November 6, 2009

Published In

Volume / Issue

  • 1216 / 45

Start / End Page

  • 7732 - 7738

PubMed ID

  • 19767005

Pubmed Central ID

  • 19767005

Electronic International Standard Serial Number (EISSN)

  • 1873-3778

Digital Object Identifier (DOI)

  • 10.1016/j.chroma.2009.08.088


  • eng

Conference Location

  • Netherlands