Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates.

Journal Article (Journal Article)

Herein we report a study combining metabolomics and mass isotopomer analysis used for investigation of the biochemical fate of γ-hydroxybutyric acid (GHB). Using various (13)C incorporation labeling patterns into GHB, we have discovered that GHB is catabolized by previously unknown processes that include (i) direct β-oxidation to acetyl-CoA and glycolate, (ii) α-oxidation to 3-hydroxypropionyl-CoA and formate, and (iii) cleavage of C-4 to yield 3-hydroxypropionate and CO2. We further utilized the unique attributes of our labeling patterns and the resultant isotopomers to quantitate relative flux down the identified pathways.

Full Text

Duke Authors

Cited Authors

  • Sadhukhan, S; Zhang, G-F; Tochtrop, GP

Published Date

  • August 15, 2014

Published In

Volume / Issue

  • 9 / 8

Start / End Page

  • 1706 - 1711

PubMed ID

  • 24933109

Pubmed Central ID

  • PMC4136706

Electronic International Standard Serial Number (EISSN)

  • 1554-8937

Digital Object Identifier (DOI)

  • 10.1021/cb500380b


  • eng

Conference Location

  • United States