Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates.
Publication
, Journal Article
Sadhukhan, S; Zhang, G-F; Tochtrop, GP
Published in: ACS Chem Biol
August 15, 2014
Herein we report a study combining metabolomics and mass isotopomer analysis used for investigation of the biochemical fate of γ-hydroxybutyric acid (GHB). Using various (13)C incorporation labeling patterns into GHB, we have discovered that GHB is catabolized by previously unknown processes that include (i) direct β-oxidation to acetyl-CoA and glycolate, (ii) α-oxidation to 3-hydroxypropionyl-CoA and formate, and (iii) cleavage of C-4 to yield 3-hydroxypropionate and CO2. We further utilized the unique attributes of our labeling patterns and the resultant isotopomers to quantitate relative flux down the identified pathways.
Duke Scholars
Published In
ACS Chem Biol
DOI
EISSN
1554-8937
Publication Date
August 15, 2014
Volume
9
Issue
8
Start / End Page
1706 / 1711
Location
United States
Related Subject Headings
- gamma-Aminobutyric Acid
- Tandem Mass Spectrometry
- Rats, Sprague-Dawley
- Rats
- Oxidation-Reduction
- Organic Chemistry
- Gas Chromatography-Mass Spectrometry
- Chromatography, Liquid
- Carboxylic Acids
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Sadhukhan, S., Zhang, G.-F., & Tochtrop, G. P. (2014). Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates. ACS Chem Biol, 9(8), 1706–1711. https://doi.org/10.1021/cb500380b
Sadhukhan, Sushabhan, Guo-Fang Zhang, and Gregory P. Tochtrop. “Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates.” ACS Chem Biol 9, no. 8 (August 15, 2014): 1706–11. https://doi.org/10.1021/cb500380b.
Sadhukhan S, Zhang G-F, Tochtrop GP. Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates. ACS Chem Biol. 2014 Aug 15;9(8):1706–11.
Sadhukhan, Sushabhan, et al. “Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates.” ACS Chem Biol, vol. 9, no. 8, Aug. 2014, pp. 1706–11. Pubmed, doi:10.1021/cb500380b.
Sadhukhan S, Zhang G-F, Tochtrop GP. Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates. ACS Chem Biol. 2014 Aug 15;9(8):1706–1711.
Published In
ACS Chem Biol
DOI
EISSN
1554-8937
Publication Date
August 15, 2014
Volume
9
Issue
8
Start / End Page
1706 / 1711
Location
United States
Related Subject Headings
- gamma-Aminobutyric Acid
- Tandem Mass Spectrometry
- Rats, Sprague-Dawley
- Rats
- Oxidation-Reduction
- Organic Chemistry
- Gas Chromatography-Mass Spectrometry
- Chromatography, Liquid
- Carboxylic Acids
- Animals