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Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates.

Publication ,  Journal Article
Sadhukhan, S; Zhang, G-F; Tochtrop, GP
Published in: ACS Chem Biol
August 15, 2014

Herein we report a study combining metabolomics and mass isotopomer analysis used for investigation of the biochemical fate of γ-hydroxybutyric acid (GHB). Using various (13)C incorporation labeling patterns into GHB, we have discovered that GHB is catabolized by previously unknown processes that include (i) direct β-oxidation to acetyl-CoA and glycolate, (ii) α-oxidation to 3-hydroxypropionyl-CoA and formate, and (iii) cleavage of C-4 to yield 3-hydroxypropionate and CO2. We further utilized the unique attributes of our labeling patterns and the resultant isotopomers to quantitate relative flux down the identified pathways.

Duke Scholars

Published In

ACS Chem Biol

DOI

EISSN

1554-8937

Publication Date

August 15, 2014

Volume

9

Issue

8

Start / End Page

1706 / 1711

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Tandem Mass Spectrometry
  • Rats, Sprague-Dawley
  • Rats
  • Oxidation-Reduction
  • Organic Chemistry
  • Gas Chromatography-Mass Spectrometry
  • Chromatography, Liquid
  • Carboxylic Acids
  • Animals
 

Citation

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MLA
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Sadhukhan, S., Zhang, G.-F., & Tochtrop, G. P. (2014). Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates. ACS Chem Biol, 9(8), 1706–1711. https://doi.org/10.1021/cb500380b
Sadhukhan, Sushabhan, Guo-Fang Zhang, and Gregory P. Tochtrop. “Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates.ACS Chem Biol 9, no. 8 (August 15, 2014): 1706–11. https://doi.org/10.1021/cb500380b.
Sadhukhan S, Zhang G-F, Tochtrop GP. Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates. ACS Chem Biol. 2014 Aug 15;9(8):1706–11.
Sadhukhan, Sushabhan, et al. “Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates.ACS Chem Biol, vol. 9, no. 8, Aug. 2014, pp. 1706–11. Pubmed, doi:10.1021/cb500380b.
Sadhukhan S, Zhang G-F, Tochtrop GP. Modular isotopomer synthesis of γ-hydroxybutyric acid for a quantitative analysis of metabolic fates. ACS Chem Biol. 2014 Aug 15;9(8):1706–1711.
Journal cover image

Published In

ACS Chem Biol

DOI

EISSN

1554-8937

Publication Date

August 15, 2014

Volume

9

Issue

8

Start / End Page

1706 / 1711

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Tandem Mass Spectrometry
  • Rats, Sprague-Dawley
  • Rats
  • Oxidation-Reduction
  • Organic Chemistry
  • Gas Chromatography-Mass Spectrometry
  • Chromatography, Liquid
  • Carboxylic Acids
  • Animals