Using isotopic tools to dissect and quantitate parallel metabolic pathways.

Published

Journal Article

4-Hydroxyacids are ubiquitous in human physiology. They are derived from the drugs of abuse gamma-hydroxybutyrate (GHB), gamma-hydroxypentanoate(GHP), in addition to the omnipresent lipid peroxidation product 4-hydroxy-2-(E)-nonenal (4-HNE). Previously we reported that 4-hydroxyacids are catabolized through two parallel pathways. In this report we detail two isotopic tools that have allowed the dissection of this catabolic process and illustrate how these tools can be used to quantify the relative flux down each pathway. We found that 4-hydroxynonanoate (4-hydroxyacid derived from 4-HNE) is primarly catabolized through a pathway that phosphorylates the C-4 hydroxyl and isomerizes it to a C-3 hydroxy compound, which is catabolized through beta-oxidation.

Full Text

Duke Authors

Cited Authors

  • Sadhukhan, S; Han, Y; Zhang, G-F; Brunengraber, H; Tochtrop, GP

Published Date

  • May 12, 2010

Published In

Volume / Issue

  • 132 / 18

Start / End Page

  • 6309 - 6311

PubMed ID

  • 20408520

Pubmed Central ID

  • 20408520

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

Digital Object Identifier (DOI)

  • 10.1021/ja100399m

Language

  • eng

Conference Location

  • United States