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Using isotopic tools to dissect and quantitate parallel metabolic pathways.

Publication ,  Journal Article
Sadhukhan, S; Han, Y; Zhang, G-F; Brunengraber, H; Tochtrop, GP
Published in: J Am Chem Soc
May 12, 2010

4-Hydroxyacids are ubiquitous in human physiology. They are derived from the drugs of abuse gamma-hydroxybutyrate (GHB), gamma-hydroxypentanoate(GHP), in addition to the omnipresent lipid peroxidation product 4-hydroxy-2-(E)-nonenal (4-HNE). Previously we reported that 4-hydroxyacids are catabolized through two parallel pathways. In this report we detail two isotopic tools that have allowed the dissection of this catabolic process and illustrate how these tools can be used to quantify the relative flux down each pathway. We found that 4-hydroxynonanoate (4-hydroxyacid derived from 4-HNE) is primarly catabolized through a pathway that phosphorylates the C-4 hydroxyl and isomerizes it to a C-3 hydroxy compound, which is catabolized through beta-oxidation.

Duke Scholars

Published In

J Am Chem Soc

DOI

EISSN

1520-5126

Publication Date

May 12, 2010

Volume

132

Issue

18

Start / End Page

6309 / 6311

Location

United States

Related Subject Headings

  • Rats
  • Metabolic Networks and Pathways
  • Liver
  • Isotopes
  • Hydroxy Acids
  • General Chemistry
  • Fatty Acids, Unsaturated
  • Animals
  • Aldehydes
  • 40 Engineering
 

Citation

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Sadhukhan, S., Han, Y., Zhang, G.-F., Brunengraber, H., & Tochtrop, G. P. (2010). Using isotopic tools to dissect and quantitate parallel metabolic pathways. J Am Chem Soc, 132(18), 6309–6311. https://doi.org/10.1021/ja100399m
Sadhukhan, Sushabhan, Yong Han, Guo-Fang Zhang, Henri Brunengraber, and Gregory P. Tochtrop. “Using isotopic tools to dissect and quantitate parallel metabolic pathways.J Am Chem Soc 132, no. 18 (May 12, 2010): 6309–11. https://doi.org/10.1021/ja100399m.
Sadhukhan S, Han Y, Zhang G-F, Brunengraber H, Tochtrop GP. Using isotopic tools to dissect and quantitate parallel metabolic pathways. J Am Chem Soc. 2010 May 12;132(18):6309–11.
Sadhukhan, Sushabhan, et al. “Using isotopic tools to dissect and quantitate parallel metabolic pathways.J Am Chem Soc, vol. 132, no. 18, May 2010, pp. 6309–11. Pubmed, doi:10.1021/ja100399m.
Sadhukhan S, Han Y, Zhang G-F, Brunengraber H, Tochtrop GP. Using isotopic tools to dissect and quantitate parallel metabolic pathways. J Am Chem Soc. 2010 May 12;132(18):6309–6311.
Journal cover image

Published In

J Am Chem Soc

DOI

EISSN

1520-5126

Publication Date

May 12, 2010

Volume

132

Issue

18

Start / End Page

6309 / 6311

Location

United States

Related Subject Headings

  • Rats
  • Metabolic Networks and Pathways
  • Liver
  • Isotopes
  • Hydroxy Acids
  • General Chemistry
  • Fatty Acids, Unsaturated
  • Animals
  • Aldehydes
  • 40 Engineering