Dynamics of glutathione and ophthalmate traced with 2H-enriched body water in rats and humans.
Published
Journal Article
We developed a LC-MS-MS assay of the (2)H labeling of free glutathione (GSH) and bound glutathione [GSSR; which includes all DTT-reducible forms, primarily glutathione disulfide (GSSG) and mixed disulfides with proteins] and ophthalmate (an index of GSH depletion) labeled from (2)H-enriched body water. In rats whose body water was 2.5% (2)H enriched for up to 31 days, GSH labeling follows a complex pattern because of different rates of labeling of its constitutive amino acids. In rats infused with [(13)C(2),(15)N-glycine]glutathione, the rate of appearance of plasma GSH was 2.1 micromol.min(-1).kg(-1), and the half-life of plasma GSH/GSSR was 6-8 min. In healthy humans whose body fluids were 0.5% (2)H enriched, the (2)H labeling of GSH/GSSR and ophthalmate can be precisely measured after 4 h, with GSH being more rapidly labeled than GSSR. Since plasma GSH/GSSR derives mostly from liver, this technique opens the way to 2) probe noninvasively the labeling pattern and redox status of the liver GSH system in humans and 2) assess the usefulness of ophthalmate as an index of GSH depletion.
Full Text
Duke Authors
Cited Authors
- Kombu, RS; Zhang, G-F; Abbas, R; Mieyal, JJ; Anderson, VE; Kelleher, JK; Sanabria, JR; Brunengraber, H
Published Date
- July 2009
Published In
Volume / Issue
- 297 / 1
Start / End Page
- E260 - E269
PubMed ID
- 19401458
Pubmed Central ID
- 19401458
Electronic International Standard Serial Number (EISSN)
- 1522-1555
Digital Object Identifier (DOI)
- 10.1152/ajpendo.00080.2009
Language
- eng
Conference Location
- United States