Skip to main content
Journal cover image

Adduct formation in quantitative bioanalysis: effect of ionization conditions on paclitaxel.

Publication ,  Journal Article
Mortier, KA; Zhang, G-F; van Peteghem, CH; Lambert, WE
Published in: J Am Soc Mass Spectrom
April 2004

Quantitative analysis of target compounds with liquid chromatography atmospheric pressure ionization mass spectrometry is sometimes hampered by adduct formation. In these situations, cationization with alkali metal ions instead of proton addition is often observed in the positive ion mode. This work studies the process of adduct formation and investigates potential strategies to control this phenomenon. Paclitaxel, a pharmaceutical chemotherapeutic agent, was used as a model compound. Electrospray (ESI), atmospheric pressure chemical ionization (APCI) and sonic spray ionization (SSI) are evaluated and compared. The work was performed on two different instruments, allowing the evaluation of different ionization behavior for different source design for electrospray, if any. Different mobile phase additives were compared, including acetic acid, formic acid, ammonium formate, and a range of primary amines. Continuous infusion was used for a fast screening, to detect optimal conditions. These were then further investigated in detail by LC-MS. The results indicate that electrospray is the more sensitive interface for this compound on the investigated apparatus. Unacceptable quantitative data were acquired without additives in the mobile phase. Generally, additives increased the reproducibility significantly. A response of mainly one ion was achieved with dodecylamine/acetic acid and acetic acid/sodium acetate. The data also point out the importance of evaluating adduct formation for compounds prone to this phenomenon during method development, especially in view of accurate quantitation.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Am Soc Mass Spectrom

DOI

ISSN

1044-0305

Publication Date

April 2004

Volume

15

Issue

4

Start / End Page

585 / 592

Location

United States

Related Subject Headings

  • Structure-Activity Relationship
  • Paclitaxel
  • Molecular Structure
  • Mass Spectrometry
  • Ions
  • Analytical Chemistry
  • Amines
  • 3401 Analytical chemistry
  • 0306 Physical Chemistry (incl. Structural)
  • 0304 Medicinal and Biomolecular Chemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mortier, K. A., Zhang, G.-F., van Peteghem, C. H., & Lambert, W. E. (2004). Adduct formation in quantitative bioanalysis: effect of ionization conditions on paclitaxel. J Am Soc Mass Spectrom, 15(4), 585–592. https://doi.org/10.1016/j.jasms.2003.12.013
Mortier, Kjell A., Guo-Fang Zhang, Carlos H. van Peteghem, and Willy E. Lambert. “Adduct formation in quantitative bioanalysis: effect of ionization conditions on paclitaxel.J Am Soc Mass Spectrom 15, no. 4 (April 2004): 585–92. https://doi.org/10.1016/j.jasms.2003.12.013.
Mortier KA, Zhang G-F, van Peteghem CH, Lambert WE. Adduct formation in quantitative bioanalysis: effect of ionization conditions on paclitaxel. J Am Soc Mass Spectrom. 2004 Apr;15(4):585–92.
Mortier, Kjell A., et al. “Adduct formation in quantitative bioanalysis: effect of ionization conditions on paclitaxel.J Am Soc Mass Spectrom, vol. 15, no. 4, Apr. 2004, pp. 585–92. Pubmed, doi:10.1016/j.jasms.2003.12.013.
Mortier KA, Zhang G-F, van Peteghem CH, Lambert WE. Adduct formation in quantitative bioanalysis: effect of ionization conditions on paclitaxel. J Am Soc Mass Spectrom. 2004 Apr;15(4):585–592.
Journal cover image

Published In

J Am Soc Mass Spectrom

DOI

ISSN

1044-0305

Publication Date

April 2004

Volume

15

Issue

4

Start / End Page

585 / 592

Location

United States

Related Subject Headings

  • Structure-Activity Relationship
  • Paclitaxel
  • Molecular Structure
  • Mass Spectrometry
  • Ions
  • Analytical Chemistry
  • Amines
  • 3401 Analytical chemistry
  • 0306 Physical Chemistry (incl. Structural)
  • 0304 Medicinal and Biomolecular Chemistry