Skip to main content

Investigation of the role of prostaglandins in nitroglycerin-induced relaxation of isolated rabbit blood vessels.

Publication ,  Journal Article
Bennett, BM; Moffat, JA; Armstrong, PW; Marks, GS
Published in: Can J Physiol Pharmacol
June 1983

The effect of inhibition of prostaglandin synthesis on nitroglycerin-induced relaxation was examined in isolated rabbit mesenteric and celiac arterial rings. An indomethacin dose of 5 microM was selected as adequate to inhibit prostaglandin synthesis as this dose prevented relaxation of the arterial rings by sodium arachidonate (3.3 microM) and by bradykinin, a peptide thought to induce vasodilation via stimulation of prostaglandin synthesis. Following 20 min pretreatment with indomethacin (5 microM), indomethacin solvent, or Krebs solution (control), the arterial rings were contracted submaximally with phenylephrine (0.5-10 microM). The degree of inhibition of phenylephrine-induced tone produced after cumulative additions of nitroglycerin (10(-10) - 5 X 10(-7) M) was assessed. In control celiac and mesenteric rings the responses to nitroglycerin were as follows: mean effective dose (ED50), 5.6 X 10(-9) +/- 4.5 X 10(-9) M (SD) and 1.1 X 10(-8) +/- 5 X 10(-9) M (SD), respectively; maximum relaxation, 97 +/- 3% (SD) and 93 +/- 7% (SD), respectively. Neither indomethacin nor indomethacin solvent affected the ED50 or maximum relaxation with nitroglycerin. We conclude that nitroglycerin-induced relaxation of rabbit celiac and mesenteric arteries appears to be mediated through a mechanism other than stimulation of prostaglandin synthesis.

Duke Scholars

Published In

Can J Physiol Pharmacol

DOI

ISSN

0008-4212

Publication Date

June 1983

Volume

61

Issue

6

Start / End Page

554 / 560

Location

Canada

Related Subject Headings

  • Vasodilation
  • Rabbits
  • Prostaglandins
  • Physiology
  • Nitroglycerin
  • Muscle, Smooth, Vascular
  • Muscle Relaxation
  • Muscle Contraction
  • Mesenteric Arteries
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bennett, B. M., Moffat, J. A., Armstrong, P. W., & Marks, G. S. (1983). Investigation of the role of prostaglandins in nitroglycerin-induced relaxation of isolated rabbit blood vessels. Can J Physiol Pharmacol, 61(6), 554–560. https://doi.org/10.1139/y83-085
Bennett, B. M., J. A. Moffat, P. W. Armstrong, and G. S. Marks. “Investigation of the role of prostaglandins in nitroglycerin-induced relaxation of isolated rabbit blood vessels.Can J Physiol Pharmacol 61, no. 6 (June 1983): 554–60. https://doi.org/10.1139/y83-085.
Bennett BM, Moffat JA, Armstrong PW, Marks GS. Investigation of the role of prostaglandins in nitroglycerin-induced relaxation of isolated rabbit blood vessels. Can J Physiol Pharmacol. 1983 Jun;61(6):554–60.
Bennett, B. M., et al. “Investigation of the role of prostaglandins in nitroglycerin-induced relaxation of isolated rabbit blood vessels.Can J Physiol Pharmacol, vol. 61, no. 6, June 1983, pp. 554–60. Pubmed, doi:10.1139/y83-085.
Bennett BM, Moffat JA, Armstrong PW, Marks GS. Investigation of the role of prostaglandins in nitroglycerin-induced relaxation of isolated rabbit blood vessels. Can J Physiol Pharmacol. 1983 Jun;61(6):554–560.

Published In

Can J Physiol Pharmacol

DOI

ISSN

0008-4212

Publication Date

June 1983

Volume

61

Issue

6

Start / End Page

554 / 560

Location

Canada

Related Subject Headings

  • Vasodilation
  • Rabbits
  • Prostaglandins
  • Physiology
  • Nitroglycerin
  • Muscle, Smooth, Vascular
  • Muscle Relaxation
  • Muscle Contraction
  • Mesenteric Arteries
  • Male