NgBR is essential for endothelial cell glycosylation and vascular development.
Journal Article (Journal Article)
NgBR is a transmembrane protein identified as a Nogo-B-interacting protein and recently has been shown to be a subunit required for cis-prenyltransferase (cisPTase) activity. To investigate the integrated role of NgBR in vascular development, we have characterized endothelial-specific NgBR knockout embryos. Here, we show that endothelial-specific NgBR knockout results in embryonic lethality due to vascular development defects in yolk sac and embryo proper. Loss of NgBR in endothelial cells reduces proliferation and promotes apoptosis of the cells largely through defects in the glycosylation of key endothelial proteins including VEGFR2, VE-cadherin, and CD31, and defective glycosylation can be rescued by treatment with the end product of cisPTase activity, dolichol phosphate. Moreover, NgBR functions in endothelial cells during embryogenesis are Nogo-B independent. These data uniquely show the importance of NgBR and protein glycosylation during vascular development.
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Duke Authors
Cited Authors
- Park, EJ; GrabiĆska, KA; Guan, Z; Sessa, WC
Published Date
- February 2016
Published In
Volume / Issue
- 17 / 2
Start / End Page
- 167 - 177
PubMed ID
- 26755743
Pubmed Central ID
- PMC5290814
Electronic International Standard Serial Number (EISSN)
- 1469-3178
Digital Object Identifier (DOI)
- 10.15252/embr.201540789
Language
- eng
Conference Location
- England