NgBR is essential for endothelial cell glycosylation and vascular development.

Published

Journal Article

NgBR is a transmembrane protein identified as a Nogo-B-interacting protein and recently has been shown to be a subunit required for cis-prenyltransferase (cisPTase) activity. To investigate the integrated role of NgBR in vascular development, we have characterized endothelial-specific NgBR knockout embryos. Here, we show that endothelial-specific NgBR knockout results in embryonic lethality due to vascular development defects in yolk sac and embryo proper. Loss of NgBR in endothelial cells reduces proliferation and promotes apoptosis of the cells largely through defects in the glycosylation of key endothelial proteins including VEGFR2, VE-cadherin, and CD31, and defective glycosylation can be rescued by treatment with the end product of cisPTase activity, dolichol phosphate. Moreover, NgBR functions in endothelial cells during embryogenesis are Nogo-B independent. These data uniquely show the importance of NgBR and protein glycosylation during vascular development.

Full Text

Duke Authors

Cited Authors

  • Park, EJ; GrabiƄska, KA; Guan, Z; Sessa, WC

Published Date

  • February 2016

Published In

Volume / Issue

  • 17 / 2

Start / End Page

  • 167 - 177

PubMed ID

  • 26755743

Pubmed Central ID

  • 26755743

Electronic International Standard Serial Number (EISSN)

  • 1469-3178

Digital Object Identifier (DOI)

  • 10.15252/embr.201540789

Language

  • eng

Conference Location

  • England