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Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa.

Publication ,  Journal Article
Thaden, JT; Lory, S; Gardner, TS
Published in: J Bacteriol
May 2010

The LasR/LasI quorum-sensing system in Pseudomonas aeruginosa influences global gene expression and mediates pathogenesis. In this study, we show that the quorum-sensing system activates, via the transcriptional regulator PA4778, a copper resistance system composed of 11 genes. The quorum-sensing global regulator LasR was recently shown to directly activate transcription of PA4778, a cueR homolog and a MerR-type transcriptional regulator. Using molecular genetic methods and bioinformatics, we verify the interaction of LasR with the PA4778 promoter and further demonstrate the LasR binding site. We also identify a putative PA4778 binding motif and show that the protein directly binds to and activates five promoters controlling the expression of 11 genes--PA3519 to -15, PA3520, mexPQ-opmE, PA3574.1, and cueA, a virulence factor in a murine model. Using gene disruptions, we show that PA4778, along with 7 of 11 gene targets of PA4778, increases the sensitivity of P. aeruginosa to elevated copper concentrations. This work identifies a cellular function for PA4778 and four other previously unannotated genes (PA3515, PA3516, PA3517, and PA3518) and suggests a potential role for copper in the quorum response. We propose to name PA4778 cueR.

Duke Scholars

Published In

J Bacteriol

DOI

EISSN

1098-5530

Publication Date

May 2010

Volume

192

Issue

10

Start / End Page

2557 / 2568

Location

United States

Related Subject Headings

  • Trans-Activators
  • Sequence Homology, Nucleic Acid
  • Quorum Sensing
  • Pseudomonas aeruginosa
  • Promoter Regions, Genetic
  • Mutation
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Microbiology
  • Gene Expression Regulation, Bacterial
 

Citation

APA
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ICMJE
MLA
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Thaden, J. T., Lory, S., & Gardner, T. S. (2010). Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa. J Bacteriol, 192(10), 2557–2568. https://doi.org/10.1128/JB.01528-09
Thaden, Joshua T., Stephen Lory, and Timothy S. Gardner. “Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa.J Bacteriol 192, no. 10 (May 2010): 2557–68. https://doi.org/10.1128/JB.01528-09.
Thaden JT, Lory S, Gardner TS. Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa. J Bacteriol. 2010 May;192(10):2557–68.
Thaden, Joshua T., et al. “Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa.J Bacteriol, vol. 192, no. 10, May 2010, pp. 2557–68. Pubmed, doi:10.1128/JB.01528-09.
Thaden JT, Lory S, Gardner TS. Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa. J Bacteriol. 2010 May;192(10):2557–2568.

Published In

J Bacteriol

DOI

EISSN

1098-5530

Publication Date

May 2010

Volume

192

Issue

10

Start / End Page

2557 / 2568

Location

United States

Related Subject Headings

  • Trans-Activators
  • Sequence Homology, Nucleic Acid
  • Quorum Sensing
  • Pseudomonas aeruginosa
  • Promoter Regions, Genetic
  • Mutation
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Microbiology
  • Gene Expression Regulation, Bacterial