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Methods for Acute and Subacute Murine Hindlimb Ischemia.

Publication ,  Journal Article
Padgett, ME; McCord, TJ; McClung, JM; Kontos, CD
Published in: J Vis Exp
June 21, 2016

Peripheral artery disease (PAD) is a leading cause of cardiovascular morbidity and mortality in developed countries, and animal models that reliably reproduce the human disease are necessary to develop new therapies for this disease. The mouse hindlimb ischemia model has been widely used for this purpose, but the standard practice of inducing acute limb ischemia by ligation of the femoral artery can result in substantial tissue necrosis, compromising investigators' ability to study the vascular and skeletal muscle tissue responses to ischemia. An alternative approach to femoral artery ligation is the induction of gradual femoral artery occlusion through the use of ameroid constrictors. When placed around the femoral artery in the same or different locations as the sites of femoral artery ligation, these devices occlude the artery over 1 - 3 days, resulting in more gradual, subacute ischemia. This results in less substantial skeletal muscle tissue necrosis, which may more closely mimic the responses seen in human PAD. Because genetic background influences outcomes in both the acute and subacute ischemia models, consideration of the mouse strain being studied is important in choosing the best model. This paper describes the proper procedure and anatomical placement of ligatures or ameroid constrictors on the mouse femoral artery to induce subacute or acute hindlimb ischemia in the mouse.

Duke Scholars

Published In

J Vis Exp

DOI

EISSN

1940-087X

Publication Date

June 21, 2016

Issue

112

Location

United States

Related Subject Headings

  • Regional Blood Flow
  • Neovascularization, Physiologic
  • Muscle, Skeletal
  • Mice
  • Ischemia
  • Hindlimb
  • Femoral Artery
  • Disease Models, Animal
  • Animals
  • 3101 Biochemistry and cell biology
 

Citation

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Padgett, M. E., McCord, T. J., McClung, J. M., & Kontos, C. D. (2016). Methods for Acute and Subacute Murine Hindlimb Ischemia. J Vis Exp, (112). https://doi.org/10.3791/54166
Padgett, Michael E., Timothy J. McCord, Joseph M. McClung, and Christopher D. Kontos. “Methods for Acute and Subacute Murine Hindlimb Ischemia.J Vis Exp, no. 112 (June 21, 2016). https://doi.org/10.3791/54166.
Padgett ME, McCord TJ, McClung JM, Kontos CD. Methods for Acute and Subacute Murine Hindlimb Ischemia. J Vis Exp. 2016 Jun 21;(112).
Padgett, Michael E., et al. “Methods for Acute and Subacute Murine Hindlimb Ischemia.J Vis Exp, no. 112, June 2016. Pubmed, doi:10.3791/54166.
Padgett ME, McCord TJ, McClung JM, Kontos CD. Methods for Acute and Subacute Murine Hindlimb Ischemia. J Vis Exp. 2016 Jun 21;(112).

Published In

J Vis Exp

DOI

EISSN

1940-087X

Publication Date

June 21, 2016

Issue

112

Location

United States

Related Subject Headings

  • Regional Blood Flow
  • Neovascularization, Physiologic
  • Muscle, Skeletal
  • Mice
  • Ischemia
  • Hindlimb
  • Femoral Artery
  • Disease Models, Animal
  • Animals
  • 3101 Biochemistry and cell biology