Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators.

Journal Article (Journal Article)

The identification of active neurons and circuits in vivo is a fundamental challenge in understanding the neural basis of behavior. Genetically encoded calcium (Ca(2+)) indicators (GECIs) enable quantitative monitoring of cellular-resolution activity during behavior. However, such indicators require online monitoring within a limited field of view. Alternatively, post hoc staining of immediate early genes (IEGs) indicates highly active cells within the entire brain, albeit with poor temporal resolution. We designed a fluorescent sensor, CaMPARI, that combines the genetic targetability and quantitative link to neural activity of GECIs with the permanent, large-scale labeling of IEGs, allowing a temporally precise "activity snapshot" of a large tissue volume. CaMPARI undergoes efficient and irreversible green-to-red conversion only when elevated intracellular Ca(2+) and experimenter-controlled illumination coincide. We demonstrate the utility of CaMPARI in freely moving larvae of zebrafish and flies, and in head-fixed mice and adult flies.

Full Text

Duke Authors

Cited Authors

  • Fosque, BF; Sun, Y; Dana, H; Yang, C-T; Ohyama, T; Tadross, MR; Patel, R; Zlatic, M; Kim, DS; Ahrens, MB; Jayaraman, V; Looger, LL; Schreiter, ER

Published Date

  • February 2015

Published In

Volume / Issue

  • 347 / 6223

Start / End Page

  • 755 - 760

PubMed ID

  • 25678659

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1260922


  • eng