Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.
Publication
, Journal Article
Matty, MA; Oehlers, SH; Tobin, DM
Published in: Methods Mol Biol
2016
Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2016
Volume
1451
Start / End Page
207 / 223
Location
United States
Related Subject Headings
- Zebrafish
- Mycobacterium marinum
- Larva
- Immunity, Innate
- Host-Pathogen Interactions
- Developmental Biology
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Matty, M. A., Oehlers, S. H., & Tobin, D. M. (2016). Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae. Methods Mol Biol, 1451, 207–223. https://doi.org/10.1007/978-1-4939-3771-4_14
Matty, Molly A., Stefan H. Oehlers, and David M. Tobin. “Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.” Methods Mol Biol 1451 (2016): 207–23. https://doi.org/10.1007/978-1-4939-3771-4_14.
Matty MA, Oehlers SH, Tobin DM. Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae. Methods Mol Biol. 2016;1451:207–23.
Matty, Molly A., et al. “Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.” Methods Mol Biol, vol. 1451, 2016, pp. 207–23. Pubmed, doi:10.1007/978-1-4939-3771-4_14.
Matty MA, Oehlers SH, Tobin DM. Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae. Methods Mol Biol. 2016;1451:207–223.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2016
Volume
1451
Start / End Page
207 / 223
Location
United States
Related Subject Headings
- Zebrafish
- Mycobacterium marinum
- Larva
- Immunity, Innate
- Host-Pathogen Interactions
- Developmental Biology
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology