Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.

Journal Article (Journal Article)

Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.

Full Text

Duke Authors

Cited Authors

  • Matty, MA; Oehlers, SH; Tobin, DM

Published Date

  • 2016

Published In

Volume / Issue

  • 1451 /

Start / End Page

  • 207 - 223

PubMed ID

  • 27464810

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-3771-4_14


  • eng

Conference Location

  • United States