Generation of a Mouse Full-length Balancer with Versatile Cassette-shuttling Selection Strategy.

Published online

Journal Article

Balancer chromosomes are important tools for a variety of genetic manipulations in lower model organisms, owing to their ability to suppress recombination. In mouse, however, such effort has not been accomplished, mostly due to the size of the chromosomes and the complexity of multiple step chromosomal engineering. We developed an effective and versatile cassette-shuttling selection (CASS) strategy involving only two selection markers to achieve the sequential production of multiple large inversions along the chromosome. Using this strategy, we successfully generated the first full-length balancer in mice and showed that Balancer 17M-GFP can efficiently suppress recombination. Our study has not only generated a useful genetic resource, but also provided a strategy for constructing mammalian balancer chromosomes.

Full Text

Duke Authors

Cited Authors

  • Ye, Z; Sun, L; Li, R; Han, M; Zhuang, Y; Wu, X; Xu, T

Published Date

  • 2016

Published In

Volume / Issue

  • 12 / 8

Start / End Page

  • 911 - 916

PubMed ID

  • 27489495

Pubmed Central ID

  • 27489495

Electronic International Standard Serial Number (EISSN)

  • 1449-2288

Digital Object Identifier (DOI)

  • 10.7150/ijbs.15172

Language

  • eng

Conference Location

  • Australia