Skip to main content
Journal cover image

Selective expression of CD44, a putative prostate cancer stem cell marker, in neuroendocrine tumor cells of human prostate cancer.

Publication ,  Journal Article
Palapattu, GS; Wu, C; Silvers, CR; Martin, HB; Williams, K; Salamone, L; Bushnell, T; Huang, L-S; Yang, Q; Huang, J
Published in: Prostate
May 15, 2009

BACKGROUND: Hormonal therapy is effective for advanced prostate cancer (PC) but the disease often recurs and becomes hormone-refractory. It is hypothesized that a subpopulation of cancer cells, that is, cancer stem cells (CSCs), survives hormonal therapy and leads to tumor recurrence. CD44 expression was shown to identify tumor cells with CSC features. PC contains secretory type epithelial cells and a minor population of neuroendocrine cells. Neuroendocrine cells do not express androgen receptor and are quiescent, features associated with CSCs. The purpose of the study was to determine the expression of CD44 in human PC and its relationship to neuroendocrine tumor cells. METHODS: Immunohistochemistry and immunofluorescence were performed to study CD44 expression in PC cell lines, single cells from fresh PC tissue and archival tissue sections of PC. We then determined if CD44+ cells represent neuroendocrine tumor cells. RESULTS: In human PC cell lines, expression of CD44 is associated with cells of NE phenotype. In human PC tissues, NE tumor cells are virtually all positive for CD44 and CD44+ cells, excluding lymphocytes, are all NE tumor cells. CONCLUSIONS: Selective expression of the stem cell-associated marker CD44 in NE tumor cells of PC, in combination with their other known features, further supports the significance of such cells in therapy resistance and tumor recurrence.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Prostate

DOI

EISSN

1097-0045

Publication Date

May 15, 2009

Volume

69

Issue

7

Start / End Page

787 / 798

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Retrospective Studies
  • Prostatic Neoplasms
  • Phosphopyruvate Hydratase
  • Oncology & Carcinogenesis
  • Neuroendocrine Tumors
  • Neoplastic Stem Cells
  • Male
  • Immunohistochemistry
  • Hyaluronan Receptors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Palapattu, G. S., Wu, C., Silvers, C. R., Martin, H. B., Williams, K., Salamone, L., … Huang, J. (2009). Selective expression of CD44, a putative prostate cancer stem cell marker, in neuroendocrine tumor cells of human prostate cancer. Prostate, 69(7), 787–798. https://doi.org/10.1002/pros.20928
Palapattu, Ganesh S., Chengyu Wu, Christopher R. Silvers, Heather B. Martin, Karin Williams, Linda Salamone, Timothy Bushnell, Li-Shan Huang, Qi Yang, and Jiaoti Huang. “Selective expression of CD44, a putative prostate cancer stem cell marker, in neuroendocrine tumor cells of human prostate cancer.Prostate 69, no. 7 (May 15, 2009): 787–98. https://doi.org/10.1002/pros.20928.
Palapattu GS, Wu C, Silvers CR, Martin HB, Williams K, Salamone L, et al. Selective expression of CD44, a putative prostate cancer stem cell marker, in neuroendocrine tumor cells of human prostate cancer. Prostate. 2009 May 15;69(7):787–98.
Palapattu, Ganesh S., et al. “Selective expression of CD44, a putative prostate cancer stem cell marker, in neuroendocrine tumor cells of human prostate cancer.Prostate, vol. 69, no. 7, May 2009, pp. 787–98. Pubmed, doi:10.1002/pros.20928.
Palapattu GS, Wu C, Silvers CR, Martin HB, Williams K, Salamone L, Bushnell T, Huang L-S, Yang Q, Huang J. Selective expression of CD44, a putative prostate cancer stem cell marker, in neuroendocrine tumor cells of human prostate cancer. Prostate. 2009 May 15;69(7):787–798.
Journal cover image

Published In

Prostate

DOI

EISSN

1097-0045

Publication Date

May 15, 2009

Volume

69

Issue

7

Start / End Page

787 / 798

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Retrospective Studies
  • Prostatic Neoplasms
  • Phosphopyruvate Hydratase
  • Oncology & Carcinogenesis
  • Neuroendocrine Tumors
  • Neoplastic Stem Cells
  • Male
  • Immunohistochemistry
  • Hyaluronan Receptors