High-speed label-free functional photoacoustic microscopy of mouse brain in action.

Journal Article (Journal Article)

We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

Full Text

Duke Authors

Cited Authors

  • Yao, J; Wang, L; Yang, J-M; Maslov, KI; Wong, TTW; Li, L; Huang, C-H; Zou, J; Wang, LV

Published Date

  • May 2015

Published In

Volume / Issue

  • 12 / 5

Start / End Page

  • 407 - 410

PubMed ID

  • 25822799

Pubmed Central ID

  • PMC4428901

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

International Standard Serial Number (ISSN)

  • 1548-7091

Digital Object Identifier (DOI)

  • 10.1038/nmeth.3336


  • eng