miR-21 improves the neurological outcome after traumatic brain injury in rats.

Journal Article

The expression levels of microRNAs (miRNAs) including miR-21, have been reported to change in response to traumatic brain injury (TBI), suggesting that they may influence the pathophysiological process in brain injury. To analyze the potential effect of miR-21 on neurological function after TBI, we employed the fluid percussion injury rat model and manipulated the expression level of miR-21 in brain using intracerebroventricular infusion of miR-21 agomir or antagomir. We found that upregulation of miR-21 level in brain conferred a better neurological outcome after TBI by improving long-term neurological function, alleviating brain edema and decreasing lesion volume. To further investigate the mechanism underlying this protective effect, we evaluated the impact of miR-21 on apoptosis and angiogenesis in brain after TBI. We found that miR-21 inhibited apoptosis and promoted angiogenesis through regulating the expression of apoptosis- and angiogenesis-related molecules. In addition, the expression of PTEN, a miR-21 target gene, was inhibited and Akt signaling was activated in the procedure. Taken together, these data indicate that miR-21 could be a potential therapeutic target for interventions after TBI.

Full Text

Duke Authors

Cited Authors

  • Ge, X-T; Lei, P; Wang, H-C; Zhang, A-L; Han, Z-L; Chen, X; Li, S-H; Jiang, R-C; Kang, C-S; Zhang, J-N

Published Date

  • January 2014

Published In

Volume / Issue

  • 4 /

Start / End Page

  • 6718 -

PubMed ID

  • 25342226

Electronic International Standard Serial Number (EISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/srep06718

Language

  • eng