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Conservation of hearing and protection of auditory hair cells against trauma-induced losses by local dexamethasone therapy: molecular and genetic mechanisms.

Publication ,  Journal Article
Van De Water, TR; Abi Hachem, RN; Dinh, CT; Bas, E; Haake, SM; Hoosien, G; Vivero, R; Chan, S; He, J; Eshraghi, AA; Angeli, SI; Telischi, FF ...
Published in: Cochlear Implants Int
June 2010

HYPOTHESIS: Dexamethasone (DXM) protects hearing against trauma-induced loss. MATERIALS: in vivo: A guinea pig model of electrode induced trauma (EIT)-induced hearing loss was used to locally deliver dexamethasone. In vitro: TNF-α-challenged organ of Corti explants treated with DXM or polymer-eluted DXM +/- PI3K/Akt/PkB/NFkB inhibitors were used for hair cells count and gene expression studies. RESULTS: in vivo: local DXM treatment of EIT-animals prevents trauma-induced loss of ABR thresholds that occurs in EIT-animals and EIT-animals treated with the carrier solution (i.e., AP), and prevented loss of auditory hair cells. In vitro: DXM and polymer-eluted DXM were equally effective in protecting hair cells from ototoxic levels of TNF-α Inhibitor treated explants demonstrated that DXM treatment requires both Akt/PKB and NFkB signalling for otoprotection. DXM treatment of explants showed up regulation of anti-apoptosis related genes (i.e., Bcl-2, Bcl-xl) and down regulation of pro-apoptosis related genes (i.e., Bax, TNFR-1). CONCLUSIONS: DXM exert its otoprotective action by activation of cell signal molecules (e.g., NFkB) that alter the expression of anti- and pro-apoptosis genes.

Duke Scholars

Published In

Cochlear Implants Int

DOI

EISSN

1754-7628

Publication Date

June 2010

Volume

11 Suppl 1

Start / End Page

42 / 55

Location

England

Related Subject Headings

  • Wounds and Injuries
  • Up-Regulation
  • Tumor Necrosis Factor-alpha
  • Reference Values
  • Real-Time Polymerase Chain Reaction
  • Random Allocation
  • Otorhinolaryngology
  • Organ of Corti
  • In Vitro Techniques
  • Hearing Loss
 

Citation

APA
Chicago
ICMJE
MLA
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Van De Water, T. R., Abi Hachem, R. N., Dinh, C. T., Bas, E., Haake, S. M., Hoosien, G., … Balkany, T. J. (2010). Conservation of hearing and protection of auditory hair cells against trauma-induced losses by local dexamethasone therapy: molecular and genetic mechanisms. Cochlear Implants Int, 11 Suppl 1, 42–55. https://doi.org/10.1179/146701010X12671178390834
Van De Water, Thomas R., Ralph N. Abi Hachem, Christine T. Dinh, Esperanza Bas, Scott M. Haake, Gia Hoosien, Richard Vivero, et al. “Conservation of hearing and protection of auditory hair cells against trauma-induced losses by local dexamethasone therapy: molecular and genetic mechanisms.Cochlear Implants Int 11 Suppl 1 (June 2010): 42–55. https://doi.org/10.1179/146701010X12671178390834.
Van De Water TR, Abi Hachem RN, Dinh CT, Bas E, Haake SM, Hoosien G, et al. Conservation of hearing and protection of auditory hair cells against trauma-induced losses by local dexamethasone therapy: molecular and genetic mechanisms. Cochlear Implants Int. 2010 Jun;11 Suppl 1:42–55.
Van De Water, Thomas R., et al. “Conservation of hearing and protection of auditory hair cells against trauma-induced losses by local dexamethasone therapy: molecular and genetic mechanisms.Cochlear Implants Int, vol. 11 Suppl 1, June 2010, pp. 42–55. Pubmed, doi:10.1179/146701010X12671178390834.
Van De Water TR, Abi Hachem RN, Dinh CT, Bas E, Haake SM, Hoosien G, Vivero R, Chan S, He J, Eshraghi AA, Angeli SI, Telischi FF, Balkany TJ. Conservation of hearing and protection of auditory hair cells against trauma-induced losses by local dexamethasone therapy: molecular and genetic mechanisms. Cochlear Implants Int. 2010 Jun;11 Suppl 1:42–55.

Published In

Cochlear Implants Int

DOI

EISSN

1754-7628

Publication Date

June 2010

Volume

11 Suppl 1

Start / End Page

42 / 55

Location

England

Related Subject Headings

  • Wounds and Injuries
  • Up-Regulation
  • Tumor Necrosis Factor-alpha
  • Reference Values
  • Real-Time Polymerase Chain Reaction
  • Random Allocation
  • Otorhinolaryngology
  • Organ of Corti
  • In Vitro Techniques
  • Hearing Loss