TBX3 Directs Cell-Fate Decision toward Mesendoderm.

Published

Journal Article

Cell-fate decisions and pluripotency are dependent on networks of key transcriptional regulators. Recent reports demonstrated additional functions of pluripotency-associated factors during early lineage commitment. The T-box transcription factor TBX3 has been implicated in regulating embryonic stem cell self-renewal and cardiogenesis. Here, we show that TBX3 is dynamically expressed during specification of the mesendoderm lineages in differentiating embryonic stem cells (ESCs) in vitro and in developing mouse and Xenopus embryos in vivo. Forced TBX3 expression in ESCs promotes mesendoderm specification by directly activating key lineage specification factors and indirectly by enhancing paracrine Nodal/Smad2 signaling. TBX3 loss-of-function analyses in the Xenopus underline its requirement for mesendoderm lineage commitment. Moreover, we uncovered a functional redundancy between TBX3 and Tbx2 during Xenopus gastrulation. Taken together, we define further facets of TBX3 actions and map TBX3 as an upstream regulator of the mesendoderm transcriptional program during gastrulation.

Full Text

Duke Authors

Cited Authors

  • Weidgang, CE; Russell, R; Tata, PR; Kühl, SJ; Illing, A; Müller, M; Lin, Q; Brunner, C; Boeckers, TM; Bauer, K; Kartikasari, AER; Guo, Y; Radenz, M; Bernemann, C; Weiß, M; Seufferlein, T; Zenke, M; Iacovino, M; Kyba, M; Schöler, HR; Kühl, M; Liebau, S; Kleger, A

Published Date

  • January 2013

Published In

Volume / Issue

  • 1 / 3

Start / End Page

  • 248 - 265

PubMed ID

  • 24319661

Pubmed Central ID

  • 24319661

Electronic International Standard Serial Number (EISSN)

  • 2213-6711

International Standard Serial Number (ISSN)

  • 2213-6711

Digital Object Identifier (DOI)

  • 10.1016/j.stemcr.2013.08.002

Language

  • eng