Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ.
Publication
, Journal Article
Chen, Y; Perfect, JR
Published in: Methods Mol Biol
2017
Multilocus sequence typing (MLST) has become the preferred method for genotyping many biological species. It can be used to identify major phylogenetic clades, molecular groups, or subpopulations of a species, as well as individual strains or clones. However, conventional MLST is costly and time consuming, which limits its power for genotyping large numbers of samples. Here, we describe a new MLST method that uses next-generation sequencing, a multiplexing protocol, and appropriate analytical software to provide accurate, rapid, and economical MLST genotyping of 96 or more isolates in a single assay.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2017
Volume
1492
Start / End Page
197 / 202
Location
United States
Related Subject Headings
- Software
- Multilocus Sequence Typing
- Genotype
- Developmental Biology
- Cost-Benefit Analysis
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Chen, Y., & Perfect, J. R. (2017). Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ. Methods Mol Biol, 1492, 197–202. https://doi.org/10.1007/978-1-4939-6442-0_14
Chen, Yuan, and John R. Perfect. “Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ.” Methods Mol Biol 1492 (2017): 197–202. https://doi.org/10.1007/978-1-4939-6442-0_14.
Chen Y, Perfect JR. Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ. Methods Mol Biol. 2017;1492:197–202.
Chen, Yuan, and John R. Perfect. “Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ.” Methods Mol Biol, vol. 1492, 2017, pp. 197–202. Pubmed, doi:10.1007/978-1-4939-6442-0_14.
Chen Y, Perfect JR. Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ. Methods Mol Biol. 2017;1492:197–202.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2017
Volume
1492
Start / End Page
197 / 202
Location
United States
Related Subject Headings
- Software
- Multilocus Sequence Typing
- Genotype
- Developmental Biology
- Cost-Benefit Analysis
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences