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Pulmonary and pleural responses in Fischer 344 rats following short-term inhalation of a synthetic vitreous fiber. II. Pathobiologic responses.

Publication ,  Journal Article
Gelzleichter, TR; Bermudez, E; Mangum, JB; Wong, BA; Moss, OR; Everitt, JI
Published in: Fundam Appl Toxicol
March 1996

The pleura is a target site for toxic effects induced by a variety of fibrous particulates, including both natural mineral and man-made vitreous fibers. We examined selected cytological and biochemical indicators of inflammation in both the pleural compartment and pulmonary parenchyma in F344 rats following inhalation of RCF-1, a kaolin-based ceramic fiber. Male F344 rats were exposed by inhalation to 89 mg/m3 (2645 WHO fibers/cc) RCF-1 6 hr/day for 5 consecutive days. In lung parenchyma, cytological and biochemical inflammatory responses occurred rapidly following exposure. In contrast, pleural responses were delayed in onset and of a much smaller magnitude than those observed in lung. At both Day 1 and Day 28 postexposure, increased quantities of lactate dehydrogenase, N-acetyl glucosaminidase, alkaline phosphatase, albumin, and neutrophils were present in bronchoalveolar lavage fluid. These responses were attenuated at the latter time point. No significant responses were detected in pleural lavage fluid until 28 days following exposure, at which time elevated numbers of macrophages and eosinophils, but not neutrophils, were observed. Increased albumin and fibronectin were also observed in PLF at this latter time point. These findings demonstrate that the onset of pleural and pulmonary responses following inhalation of RCF-1 are temporally separated and that pleural injury may increase in severity with time following exposure. The increase in severity of pleural inflammation found in the postexposure period cannot be readily explained by fiber translocation.

Duke Scholars

Published In

Fundam Appl Toxicol

DOI

ISSN

0272-0590

Publication Date

March 1996

Volume

30

Issue

1

Start / End Page

39 / 46

Location

United States

Related Subject Headings

  • Toxicology
  • Rats, Inbred F344
  • Rats
  • Pleura
  • Mineral Fibers
  • Male
  • Lung
  • Leukocytes
  • Kaolin
  • Glass
 

Citation

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MLA
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Gelzleichter, T. R., Bermudez, E., Mangum, J. B., Wong, B. A., Moss, O. R., & Everitt, J. I. (1996). Pulmonary and pleural responses in Fischer 344 rats following short-term inhalation of a synthetic vitreous fiber. II. Pathobiologic responses. Fundam Appl Toxicol, 30(1), 39–46. https://doi.org/10.1006/faat.1996.0041
Gelzleichter, T. R., E. Bermudez, J. B. Mangum, B. A. Wong, O. R. Moss, and J. I. Everitt. “Pulmonary and pleural responses in Fischer 344 rats following short-term inhalation of a synthetic vitreous fiber. II. Pathobiologic responses.Fundam Appl Toxicol 30, no. 1 (March 1996): 39–46. https://doi.org/10.1006/faat.1996.0041.
Gelzleichter TR, Bermudez E, Mangum JB, Wong BA, Moss OR, Everitt JI. Pulmonary and pleural responses in Fischer 344 rats following short-term inhalation of a synthetic vitreous fiber. II. Pathobiologic responses. Fundam Appl Toxicol. 1996 Mar;30(1):39–46.
Gelzleichter, T. R., et al. “Pulmonary and pleural responses in Fischer 344 rats following short-term inhalation of a synthetic vitreous fiber. II. Pathobiologic responses.Fundam Appl Toxicol, vol. 30, no. 1, Mar. 1996, pp. 39–46. Pubmed, doi:10.1006/faat.1996.0041.
Gelzleichter TR, Bermudez E, Mangum JB, Wong BA, Moss OR, Everitt JI. Pulmonary and pleural responses in Fischer 344 rats following short-term inhalation of a synthetic vitreous fiber. II. Pathobiologic responses. Fundam Appl Toxicol. 1996 Mar;30(1):39–46.

Published In

Fundam Appl Toxicol

DOI

ISSN

0272-0590

Publication Date

March 1996

Volume

30

Issue

1

Start / End Page

39 / 46

Location

United States

Related Subject Headings

  • Toxicology
  • Rats, Inbred F344
  • Rats
  • Pleura
  • Mineral Fibers
  • Male
  • Lung
  • Leukocytes
  • Kaolin
  • Glass