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Autocrine growth stimulation by transforming growth factor alpha in asbestos-transformed rat mesothelial cells.

Publication ,  Journal Article
Walker, C; Everitt, J; Ferriola, PC; Stewart, W; Mangum, J; Bermudez, E
Published in: Cancer Res
February 1, 1995

Although the association between asbestos exposure and mesothelioma development has been established for decades, very little is known regarding the molecular mechanism(s) by which asbestos fibers induce this disease. In this series of experiments, the potential for transforming growth factor alpha (TGF-alpha) to act as an autocrine growth factor in transformed mesothelial cells was examined in rats, a model system frequently used to assess the tumorigenic potential of fibrous particulates. Both asbestos-transformed cells and spontaneously transformed cells expressed functional EGF receptors, although only the asbestos-transformed cells expressed TGF-alpha. Expression of TGF-alpha transcripts was correlated with secretion of picogram amounts of growth factor into conditioned medium by the asbestos-transformed cells. In addition, whereas TGF-alpha inhibited the growth of spontaneously transformed mesothelial cells, it stimulated the growth of asbestos-transformed cells. Neutralizing antibody that recognized TGF-alpha secreted by the asbestos-transformed cells was able to inhibit the growth of these cells. Taken together, these data indicate that TGF-alpha acts as an autocrine growth factor for asbestos-transformed rat mesothelial cells. Therefore, in asbestos-transformed mesothelial cells, altered production and responsiveness to TGF-alpha distinguish these cells from spontaneously transformed mesothelial cells. These data suggest that differences in mesothelioma etiology may be reflected in differences in the molecular alterations present in these tumors.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

February 1, 1995

Volume

55

Issue

3

Start / End Page

530 / 536

Location

United States

Related Subject Headings

  • Tyrosine
  • Tumor Cells, Cultured
  • Transforming Growth Factor alpha
  • Rats
  • Radioimmunoassay
  • Phosphotyrosine
  • Phosphoproteins
  • Peritoneal Neoplasms
  • Oncology & Carcinogenesis
  • Mesothelioma
 

Citation

APA
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ICMJE
MLA
NLM
Walker, C., Everitt, J., Ferriola, P. C., Stewart, W., Mangum, J., & Bermudez, E. (1995). Autocrine growth stimulation by transforming growth factor alpha in asbestos-transformed rat mesothelial cells. Cancer Res, 55(3), 530–536.
Walker, C., J. Everitt, P. C. Ferriola, W. Stewart, J. Mangum, and E. Bermudez. “Autocrine growth stimulation by transforming growth factor alpha in asbestos-transformed rat mesothelial cells.Cancer Res 55, no. 3 (February 1, 1995): 530–36.
Walker C, Everitt J, Ferriola PC, Stewart W, Mangum J, Bermudez E. Autocrine growth stimulation by transforming growth factor alpha in asbestos-transformed rat mesothelial cells. Cancer Res. 1995 Feb 1;55(3):530–6.
Walker, C., et al. “Autocrine growth stimulation by transforming growth factor alpha in asbestos-transformed rat mesothelial cells.Cancer Res, vol. 55, no. 3, Feb. 1995, pp. 530–36.
Walker C, Everitt J, Ferriola PC, Stewart W, Mangum J, Bermudez E. Autocrine growth stimulation by transforming growth factor alpha in asbestos-transformed rat mesothelial cells. Cancer Res. 1995 Feb 1;55(3):530–536.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

February 1, 1995

Volume

55

Issue

3

Start / End Page

530 / 536

Location

United States

Related Subject Headings

  • Tyrosine
  • Tumor Cells, Cultured
  • Transforming Growth Factor alpha
  • Rats
  • Radioimmunoassay
  • Phosphotyrosine
  • Phosphoproteins
  • Peritoneal Neoplasms
  • Oncology & Carcinogenesis
  • Mesothelioma