Skip to main content

Characterization of platelet-derived growth factor and platelet-derived growth factor receptor expression in asbestos-induced rat mesothelioma.

Publication ,  Journal Article
Walker, C; Bermudez, E; Stewart, W; Bonner, J; Molloy, CJ; Everitt, J
Published in: Cancer Res
January 15, 1992

Although altered expression of platelet-derived growth factor (PDGF) is a hallmark of human mesothelioma, expression of PDGF receptors has not been characterized in this cell type. In addition, the expression of this growth factor and its cognate receptor in rodent mesothelioma has not been investigated. In this study, examination of transformed mesothelial cells derived from asbestos-induced rat mesotheliomas revealed that these cells expressed high affinity PDGF receptors (Kd = 0.5 nM) and receptor number was 1.6 x 10(5)/cell. Western analysis using antibodies specific for either the alpha-type or beta-type PDGF receptor and Northern analysis using probes specific for alpha- and beta-type receptor RNA transcripts indicated that these cells expressed beta-type PDGF receptors but that alpha-type receptors could not be detected. However, when the mesothelioma-derived cells were examined for the expression of PDGF, no expression of this growth factor could be detected. The transformed cells expressed no detectable A- or B-chain PDGF RNA transcripts; and using a competitive enzyme immunoassay specific for isoforms containing the B chain of PDGF and a sandwich enzyme-linked immunosorbent assay specific for A-chain-containing isoforms, neither AA, nor AB, nor BB isoforms of this growth factor could be detected in medium conditioned by these cells. The absence of alterations in PDGF expression in rat mesothelioma, in contrast to the data for the human disease, suggests that the production of this growth factor by transformed mesothelial cells may be species specific.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

January 15, 1992

Volume

52

Issue

2

Start / End Page

301 / 306

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Platelet-Derived Growth Factor
  • Receptors, Cell Surface
  • Rats
  • RNA, Neoplasm
  • RNA, Messenger
  • Platelet-Derived Growth Factor
  • Oncology & Carcinogenesis
  • Mesothelioma
  • Gene Expression
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Walker, C., Bermudez, E., Stewart, W., Bonner, J., Molloy, C. J., & Everitt, J. (1992). Characterization of platelet-derived growth factor and platelet-derived growth factor receptor expression in asbestos-induced rat mesothelioma. Cancer Res, 52(2), 301–306.
Walker, C., E. Bermudez, W. Stewart, J. Bonner, C. J. Molloy, and J. Everitt. “Characterization of platelet-derived growth factor and platelet-derived growth factor receptor expression in asbestos-induced rat mesothelioma.Cancer Res 52, no. 2 (January 15, 1992): 301–6.
Walker C, Bermudez E, Stewart W, Bonner J, Molloy CJ, Everitt J. Characterization of platelet-derived growth factor and platelet-derived growth factor receptor expression in asbestos-induced rat mesothelioma. Cancer Res. 1992 Jan 15;52(2):301–6.
Walker C, Bermudez E, Stewart W, Bonner J, Molloy CJ, Everitt J. Characterization of platelet-derived growth factor and platelet-derived growth factor receptor expression in asbestos-induced rat mesothelioma. Cancer Res. 1992 Jan 15;52(2):301–306.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

January 15, 1992

Volume

52

Issue

2

Start / End Page

301 / 306

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Platelet-Derived Growth Factor
  • Receptors, Cell Surface
  • Rats
  • RNA, Neoplasm
  • RNA, Messenger
  • Platelet-Derived Growth Factor
  • Oncology & Carcinogenesis
  • Mesothelioma
  • Gene Expression