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Trisomy of rat chromosome 1 associated with mesothelial cell transformation.

Publication ,  Journal Article
Funaki, K; Everitt, J; Bermudez, E; Walker, C
Published in: Cancer Res
August 1, 1991

Identification of specific chromosomal aberrations in transformed mesothelial cells is an important step in elucidating the mechanism of transformation of these cells which are targets for occupational and environmental carcinogens, such as asbestos fibers. Cytogenetic analysis of normal rat mesothelial cell lines revealed that at late passage (p20-p34), trisomy of chromosome 1 was present in greater than 80% of the cells in four spontaneously immortalized lines examined, whereas at early passage (p8-p10), only 15-44% of the cells had trisomy 1. Trisomy of chromosome 1 had increased in the population as a function of passage, suggesting that cells with trisomy 1 had a selective growth advantage under in vitro culture conditions and that this alteration was associated with transformation. A commercially available rat mesothelial cell line (4/4 RM4, ATCC), was also found to have a duplication of a portion of the long arm of chromosome 1. To determine if chromosome 1 alterations have relevance to the transformed phenotype in vivo, a neoplastic cell line was established from a spontaneous rat mesothelioma. At passage 15, trisomy of chromosome 1 was observed in 26% of the metaphases in this line. However, when these cells were injected into nude mice, 99% of the cells from the resulting tumor contained an additional copy of chromosome 1. Therefore, trisomy 1 also conferred a selective growth advantage in vivo and/or was associated with the malignant subpopulation in the tumor derived cell line. These studies suggest that chromosome 1 contains a gene(s) involved in transformation of rat mesothelial cells.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

August 1, 1991

Volume

51

Issue

15

Start / End Page

4059 / 4066

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Trisomy
  • Rats
  • Pleura
  • Oncology & Carcinogenesis
  • Mesothelioma
  • Karyotyping
  • Epithelium
  • Epithelial Cells
  • Chromosomes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Funaki, K., Everitt, J., Bermudez, E., & Walker, C. (1991). Trisomy of rat chromosome 1 associated with mesothelial cell transformation. Cancer Res, 51(15), 4059–4066.
Funaki, K., J. Everitt, E. Bermudez, and C. Walker. “Trisomy of rat chromosome 1 associated with mesothelial cell transformation.Cancer Res 51, no. 15 (August 1, 1991): 4059–66.
Funaki K, Everitt J, Bermudez E, Walker C. Trisomy of rat chromosome 1 associated with mesothelial cell transformation. Cancer Res. 1991 Aug 1;51(15):4059–66.
Funaki, K., et al. “Trisomy of rat chromosome 1 associated with mesothelial cell transformation.Cancer Res, vol. 51, no. 15, Aug. 1991, pp. 4059–66.
Funaki K, Everitt J, Bermudez E, Walker C. Trisomy of rat chromosome 1 associated with mesothelial cell transformation. Cancer Res. 1991 Aug 1;51(15):4059–4066.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

August 1, 1991

Volume

51

Issue

15

Start / End Page

4059 / 4066

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Trisomy
  • Rats
  • Pleura
  • Oncology & Carcinogenesis
  • Mesothelioma
  • Karyotyping
  • Epithelium
  • Epithelial Cells
  • Chromosomes