Scholars@Duke publication: G protein-coupled receptor 56 regulates mechanical overload-induced muscle hypertrophy.

G protein-coupled receptor 56 regulates mechanical overload-induced muscle hypertrophy.

Publication , Journal Article

White, JP; Wrann, CD; Rao, RR; Nair, SK; Jedrychowski, MP; You, J-S; Martínez-Redondo, V; Gygi, SP; Ruas, JL; Hornberger, TA; Wu, Z; Glass, DJ ...

Published in: Proc Natl Acad Sci U S A

November 4, 2014

Published version (DOI) Link to item

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4 (PGC-1α4) is a protein isoform derived by alternative splicing of the PGC1α mRNA and has been shown to promote muscle hypertrophy. We show here that G protein-coupled receptor 56 (GPR56) is a transcriptional target of PGC-1α4 and is induced in humans by resistance exercise. Furthermore, the anabolic effects of PGC-1α4 in cultured murine muscle cells are dependent on GPR56 signaling, because knockdown of GPR56 attenuates PGC-1α4-induced muscle hypertrophy in vitro. Forced expression of GPR56 results in myotube hypertrophy through the expression of insulin-like growth factor 1, which is dependent on Gα12/13 signaling. A murine model of overload-induced muscle hypertrophy is associated with increased expression of both GPR56 and its ligand collagen type III, whereas genetic ablation of GPR56 expression attenuates overload-induced muscle hypertrophy and associated anabolic signaling. These data illustrate a signaling pathway through GPR56 which regulates muscle hypertrophy associated with resistance/loading-type exercise.

Duke Scholars

Author James Patrick White Jr. Medicine, Hematology

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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.1417898111

EISSN

1091-6490

Publication Date

November 4, 2014

Volume

111

Issue

Start / End Page

15756 / 15761

Location

United States

Related Subject Headings

Transcription Factors
Signal Transduction
Receptors, G-Protein-Coupled
Physical Conditioning, Animal
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Muscle Proteins
Muscle Fibers, Skeletal
Mice
Male
Insulin-Like Growth Factor I

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Chicago

ICMJE

MLA

NLM

White, J. P., Wrann, C. D., Rao, R. R., Nair, S. K., Jedrychowski, M. P., You, J.-S., … Spiegelman, B. M. (2014). G protein-coupled receptor 56 regulates mechanical overload-induced muscle hypertrophy. Proc Natl Acad Sci U S A, 111(44), 15756–15761. https://doi.org/10.1073/pnas.1417898111

White, James P., Christiane D. Wrann, Rajesh R. Rao, Sreekumaran K. Nair, Mark P. Jedrychowski, Jae-Sung You, Vicente Martínez-Redondo, et al. “G protein-coupled receptor 56 regulates mechanical overload-induced muscle hypertrophy.” Proc Natl Acad Sci U S A 111, no. 44 (November 4, 2014): 15756–61. https://doi.org/10.1073/pnas.1417898111.

White JP, Wrann CD, Rao RR, Nair SK, Jedrychowski MP, You J-S, et al. G protein-coupled receptor 56 regulates mechanical overload-induced muscle hypertrophy. Proc Natl Acad Sci U S A. 2014 Nov 4;111(44):15756–61.

White, James P., et al. “G protein-coupled receptor 56 regulates mechanical overload-induced muscle hypertrophy.” Proc Natl Acad Sci U S A, vol. 111, no. 44, Nov. 2014, pp. 15756–61. Pubmed, doi:10.1073/pnas.1417898111.

White JP, Wrann CD, Rao RR, Nair SK, Jedrychowski MP, You J-S, Martínez-Redondo V, Gygi SP, Ruas JL, Hornberger TA, Wu Z, Glass DJ, Piao X, Spiegelman BM. G protein-coupled receptor 56 regulates mechanical overload-induced muscle hypertrophy. Proc Natl Acad Sci U S A. 2014 Nov 4;111(44):15756–15761.

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.1417898111

EISSN

1091-6490

Publication Date

November 4, 2014

Volume

111

Issue

Start / End Page

15756 / 15761

Location

United States

Related Subject Headings

Transcription Factors
Signal Transduction
Receptors, G-Protein-Coupled
Physical Conditioning, Animal
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Muscle Proteins
Muscle Fibers, Skeletal
Mice
Male
Insulin-Like Growth Factor I