Outer membrane of avian myeloblastosis virus.

Published

Journal Article

Guinea pigs immunized intracerebrally with avian myeloblastosis virus (AMV) produced antiserum which reacted with intact virus particles in complement fixation. The antigen in question appeared to be located on the surface of the virion and could be distinguished from the type-specific virus envelope and the group-specific internal antigens of chicken leukosis-sarcoma viruses (ChiLSV). The material could be isolated by sequential treatments of AMV with bromelin, Tween 20, and freeze-thawing, and could be purified by differential centrifugation. Electron microscopy analysis indicated the presence of a component resembling the outer membrane of the particle. The antigenic determinant was designated virus membrane antigen (Vm). Further analyses revealed the presence of protein, lipid, and carbohydrate in a material having a molecular weight of about 6,000 as determined by sodium dodecyl sulfate gel electrophoresis. Serological studies suggested that the outer membranes of AMV and other ChiLSV are represented mainly by host cellular material.

Full Text

Duke Authors

Cited Authors

  • Ishizaki, R; Luftig, RB; Bolognesi, DP

Published Date

  • December 1, 1973

Published In

Volume / Issue

  • 12 / 6

Start / End Page

  • 1579 - 1588

PubMed ID

  • 4128385

Pubmed Central ID

  • 4128385

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States