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Cumulative exposure assessment for trace-level polycyclic aromatic hydrocarbons (PAHs) using human blood and plasma analysis.

Publication ,  Journal Article
Pleil, JD; Stiegel, MA; Sobus, JR; Tabucchi, S; Ghio, AJ; Madden, MC
Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
July 2010

Humans experience chronic cumulative trace-level exposure to mixtures of volatile, semi-volatile, and non-volatile polycyclic aromatic hydrocarbons (PAHs) present in the environment as by-products of combustion processes. Certain PAHs are known or suspected human carcinogens and so we have developed methodology for measuring their circulating (blood borne) concentrations as a tool to assess internal dose and health risk. We use liquid/liquid extraction and gas chromatography-mass spectrometry and present analytical parameters including dynamic range (0-250 ng/ml), linearity (>0.99 for all compounds), and instrument sensitivity (range 2-22 pg/ml) for a series of 22 PAHs representing 2-6-rings. The method is shown to be sufficiently sensitive for estimating PAHs baseline levels (typical median range from 1 to 1000 pg/ml) in groups of normal control subjects using 1-ml aliquots of human plasma but we note that some individuals have very low background concentrations for 5- and 6-ring compounds that fall below robust quantitation levels.

Duke Scholars

Published In

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI

EISSN

1873-376X

ISSN

1570-0232

Publication Date

July 2010

Volume

878

Issue

21

Start / End Page

1753 / 1760

Related Subject Headings

  • Regression Analysis
  • Polycyclic Aromatic Hydrocarbons
  • Humans
  • Hexanes
  • Gas Chromatography-Mass Spectrometry
  • Freezing
  • Environmental Exposure
  • Chemical Fractionation
  • 3401 Analytical chemistry
  • 3214 Pharmacology and pharmaceutical sciences
 

Citation

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ICMJE
MLA
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Pleil, J. D., Stiegel, M. A., Sobus, J. R., Tabucchi, S., Ghio, A. J., & Madden, M. C. (2010). Cumulative exposure assessment for trace-level polycyclic aromatic hydrocarbons (PAHs) using human blood and plasma analysis. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(21), 1753–1760. https://doi.org/10.1016/j.jchromb.2010.04.035
Pleil, J. D., M. A. Stiegel, J. R. Sobus, S. Tabucchi, A. J. Ghio, and M. C. Madden. “Cumulative exposure assessment for trace-level polycyclic aromatic hydrocarbons (PAHs) using human blood and plasma analysis.Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 878, no. 21 (July 2010): 1753–60. https://doi.org/10.1016/j.jchromb.2010.04.035.
Pleil JD, Stiegel MA, Sobus JR, Tabucchi S, Ghio AJ, Madden MC. Cumulative exposure assessment for trace-level polycyclic aromatic hydrocarbons (PAHs) using human blood and plasma analysis. Journal of chromatography B, Analytical technologies in the biomedical and life sciences. 2010 Jul;878(21):1753–60.
Pleil, J. D., et al. “Cumulative exposure assessment for trace-level polycyclic aromatic hydrocarbons (PAHs) using human blood and plasma analysis.Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 21, July 2010, pp. 1753–60. Epmc, doi:10.1016/j.jchromb.2010.04.035.
Pleil JD, Stiegel MA, Sobus JR, Tabucchi S, Ghio AJ, Madden MC. Cumulative exposure assessment for trace-level polycyclic aromatic hydrocarbons (PAHs) using human blood and plasma analysis. Journal of chromatography B, Analytical technologies in the biomedical and life sciences. 2010 Jul;878(21):1753–1760.
Journal cover image

Published In

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI

EISSN

1873-376X

ISSN

1570-0232

Publication Date

July 2010

Volume

878

Issue

21

Start / End Page

1753 / 1760

Related Subject Headings

  • Regression Analysis
  • Polycyclic Aromatic Hydrocarbons
  • Humans
  • Hexanes
  • Gas Chromatography-Mass Spectrometry
  • Freezing
  • Environmental Exposure
  • Chemical Fractionation
  • 3401 Analytical chemistry
  • 3214 Pharmacology and pharmaceutical sciences